空间结构,化学反应和定数感应塑造了复杂的多孔介质中的细菌生物质积累
David Scheidweiler1, Ankur Deep Bordoloi2, Wenqiao Jiao2
1Institute of Earth Sciences, University of Lausanne, CH-1015, Lausanne, Switzerland. david.scheidweiler@gmail.com.
Nature communications
|January 3, 2024
概括
流量和质量感应指导大肠杆菌在复杂的多孔环境中的殖民. 细菌的交流和移动导致生物质的积累和逃离结构化系统中的枯竭区域.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 环境工程 环境工程
背景情况:
- 生物组织和工程系统具有复杂的多孔结构,影响流体流动和微生物殖民.
- 了解生物和非生物因素如何相互作用以控制这些异质环境中的细菌生物质积累仍然具有挑战性.
研究的目的:
- 研究流媒体相互作用,细菌定量感应 (QS) 和化学反应如何控制大肠杆菌 (E. coli) 对多孔结构的殖民.
- 使用死角毛孔 (DEP) 和传导毛孔 (TP) 建模肠状环境,以了解结构化介质中的细菌行为.
主要方法:
- 使用了一种具有异质多孔结构 (DEP和TP) 的微流体系统,模仿哺乳动物肠道表面.
- 引入大肠杆菌和操纵液体流动以观察殖民模式.
- 分析了在细菌积累和聚合物形成中,定数感应信号分子自导体-2 (AI-2) 和化学反应的作用.
主要成果:
- 流量和AI-2梯度促进了大肠杆菌在DEP中的化学性积累.
- 在DEP中拥挤的条件导致由于生长和细胞碰撞而形成悬浮细菌聚合物.
- 形成了资源消耗热点,引发了生物质从营养和氧气贫乏的DEP机械逃逸.
结论:
- 微尺度结构,复杂的流量,细菌定数感应和化学反应共同控制异质细菌生物质的积累.
- 这些发现对了解肠道,土壤和过器等各种环境中的微生物群落有影响.
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