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在质母细胞瘤发育中的HSV1微RNA:一个in silico研究

Mahdi Abdoli Shadbad¹, Nima Hemmat^2,3, Mahla Abdoli Shadbad²

¹Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.

Scientific reports

|January 3, 2024

概括

这项研究揭示了特定的单纯疹病毒1型 (HSV1) 微RNA (miRs) 在多种质母细胞瘤 (GBM) 中被上调,可能通过降低关键基因的调节来驱动瘤的发展. 这些发现为GBM病原体和HSV1-miR角色提供了新的见解.

科学领域:

* 瘤学在线咨询
* 病毒学病毒学
* 生物信息学是一门学科.

背景情况:

*多形质母细胞瘤 (GBM) 是一种具有攻击性的原发性脑瘤.
*病毒微RNAs (miRs) 调节基因表达,但它们在GBM中的作用尚未被探索.
*简单疹病毒1型 (HSV1) 编码miRs并影响中枢神经系统.

研究的目的:

* 调查HSV1编码的miRs在GBM发展中的参与.
* 确定这些病毒miRs如何调节GBM中的瘤抑制和瘤基因表达.

主要方法:

*对公共数据集进行生物信息分析 (GSE158284,GSE153679,GSE182109).
*使用R.中的limma包的差异基因表达分析.
* 单细胞RNA测序分析使用R.R.中的Seurat包.
*TCGA-GTEX,GDSC,CTRP,免疫遗传学和丰富性分析. 在这些分析中,

主要成果:

*HSV1编码的miRs hsv1-miR-H6-3p和hsv1-miR-H1-5p在GBM中进行上调.
* hsv1-miR-H6-3p降低了GBM组织中EPB41L1和SH3PXD2A的表达.
*hsv1-miR-H1-5p降低了MELK,FZD2,NOVA1,TMEM97,PTPRZ1和GBM中的PDGFC的表达.
* 这些向基因在GBM微环境中的星细胞中表达.

结论:

*HSV1 miRs在GBM病原发生过程中起着重要作用.
*高调的HSV1 miRs通过改变瘤基因和瘤抑制剂表达来促进GBM的发展.
*为进一步研究GBM中HSV1 miRs提供了基础.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...