综合整体转录组剖析揭示了结直肠癌中基于RNA的复杂的循环RNA竞争性内源RNA调节网络
Hasan Mollanoori1, Yaser Ghelmani2, Bita Hassani3
1Medical Genetics Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Scientific reports
|January 3, 2024
概括
循环RNAs (circRNAs) 调节癌症中的基因表达. 这项研究确定了与结直肠癌相关的circRNA及其调节网络,揭示了潜在的诊断和治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- circRNAs可以充当miRNA海绵,影响基因表达,影响癌症的发病和进展.
研究的目的:
- 为了确定与结直肠癌 (CRC) 相关的circRNAs.
- 在CRC病变发生过程中阐明circRNA/miRNA/mRNA网络的分子机制.
- 发现CRC诊断和治疗的潜在生物标志物.
主要方法:
- 从GEO微阵列数据集中分析差异表达的circRNAs,miRNAs和mRNAs.
- 使用Cytoscape构建circRNA/miRNA/mRNA ceRNA网络.
- 使用TCGA和GEO数据进行功能丰富分析,生存分析和ROC曲线分析.
主要成果:
- 在CRC中确定了23个上调和31个下调的circRNAs.
- 构建了复杂的ceRNA网络,突出了特定的circRNAs (hsa_circ_0001955, hsa_circ_0071681).
- 确定了一个ceRNA子网络,涉及这些circRNAs调节与生存相关的基因 (KLF4,MYC,CCNA2,RACGAP1,CD44).
结论:
- 在CRC建立了一个全面的监管网络.
- 鉴定的circRNA及其网络为新的CRC诊断,预后和治疗策略提供了潜力.
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