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蛋白质基因分析识别了与快速进展的间歇性肺病相关的SPP1在抗MDA5阳性皮肤肌炎中

Yulu Qiu¹, Xiaoke Feng^2,3, Chang Liu¹

¹Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, China.

Arthritis research & therapy

|January 3, 2024

概括

与抗黑色素瘤分化相关的5基因抗体阳性皮肤肌炎 (MDA5+ DM) 患者患有快速进展的间歇性肺病 (RP-ILD) 显示血蛋白升高. 分泌的蛋白1 (SPP1) 可能作为RP-ILD的早期生物标志物.

科学领域:

免疫学免疫学免疫学
肺部病理学肺部病理学
蛋白质组学是指蛋白质组学.

背景情况:

与抗黑色素瘤分化相关的5基因抗体阳性皮肤肌炎 (MDA5+DM) 与快速进展的间歇性肺病 (RP-ILD) 密切相关.
在MDA5+DM患者中早期诊断RP-ILD具有挑战性.
在这个患者群体中确定RP-ILD的预后因素至关重要.

研究的目的:

确定和验证RP-ILD在MDA5+DM患者中的预后因素.
为了研究MDA5+DM患者与没有RP-ILD之间的蛋白质差异.
探索潜在的血生物标志物用于早期RP-ILD检测.

主要方法:

从MDA5+DM患者和健康对照组采集的血样本的蛋白质组分析,使用液体染色学-并联质谱法 (LC-MS/MS).
使用酶相关免疫吸收试验 (ELISA) 在独立队列中验证候选蛋白质生物标记物.
在RP-ILD和非RP-ILD患者组之间比较蛋白质表达水平.

主要成果:

在MDA5+DM患者和健康对照人群中发现了413种差异表达蛋白 (DEP).
在RP-ILD患者中,79种蛋白质发生了变化,表明炎症,凝血和补充通路的参与.
在RP-ILD患者中,分泌的脂蛋白1 (SPP1),血清粉胺A1 (SAA1) 和基尼诺基因1 (KNG1) 显著升高;在高风险RP-ILD亚组中,SPP1特别高.

结论:

这项研究为MDA5+ DM中RP-ILD的发病过程提供了新的见解.
血蛋白SPP1显示出作为RP-ILD在MDA5+DM患者中早期预警生物标志物的潜力.
需要进一步的研究来验证SPP1在RP-ILD风险分层中的临床使用.

关键词:

生物标志物生物标志物皮肤肌肉菌性炎 (dermatomyositis) 是一种疾病.间歇性肺病间歇性肺病 MDA5 MDA5 MDA5 的意思是什么意思在 RP-ILD 的基础上.

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