智能格子光片显微镜用于成像罕见和复杂的细胞事件.
Yu Shi1, Jimmy S Tabet1, Daniel E Milkie2
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Nature methods
|January 3, 2024
概括
智能显微镜与人工智能 (AI) 自主切换成像模式以捕捉罕见的细胞事件. 这使得细胞分裂和免疫突触形成的详细分析能够以前所未有的速度和规模进行.
科学领域:
- 细胞生物学 细胞生物学
- 显微镜的使用方法
- 免疫学 免疫学 免疫学
背景情况:
- 生物过程发生在不同的时空尺度上.
- 捕捉罕见或短暂的事件是单一成像模式的挑战.
- 长期的细胞表型可以源于短暂的生物事件.
研究的目的:
- 开发一个人工智能驱动的显微镜系统,用于自主切换成像技术.
- 为了克服捕捉罕见生物事件的局限性.
- 为了实现复杂的细胞动态的高分辨率,长期成像.
主要方法:
- 开发smartLLSM,一个集成AI用于仪器控制的显微镜.
- 在光反向成像和晶格光片显微镜 (LLSM) 之间自主切换.
- 在异质细胞群中研究细胞分裂和免疫突触形成的应用.
主要成果:
- smartLLSM提供了成千上万个细胞的人口级统计数据.
- 自动捕获多色3D数据集和罕见事件的4D时间缩短电影.
- 塔克索对细胞分裂和抗原强度对免疫突触的量化影响.
结论:
- 智能LLSM有效地检测和记录异质细胞群中的罕见事件.
- 在统计学上显著的复制品上具有高的时空4D成像能力.
- 能够对以前难以捕捉的细胞过程进行详细分析.
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