在一个类似胚胎的培养中获得人类原始生殖细胞样细胞的衍生

在一个类似胚胎的培养中获得人类原始生殖细胞样细胞的衍生

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Updated: Jul 6, 2025

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

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Sajedeh Nasr Esfahani¹, Yi Zheng^1,2, Auriana Arabpour^3,4,5

¹Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.

Nature communications

|January 3, 2024

概括

研究人员使用生物模拟干细胞培养诱导了人类原始生殖细胞样细胞 (hPGCLCs). 这一突破促进了对人类早期发育的理解,并为生殖医学应用提供了潜在的机会.

科学领域:

发展生物学发展生物学
干细胞生物学干细胞生物学
生殖医学生殖医学

背景情况:

原始生殖细胞 (PGCs) 对于将遗传信息传递到代后代至关重要.
胚胎发育的缺陷与不孕症有关,并影响人类进化研究.
人类PGC (hPGC) 规范的了解很少,这阻碍了生殖医学的进步.

研究的目的:

开发一种生物模拟培养系统,用于诱导人类PGC类细胞 (hPGCLCs).
阐明 hPGCLC 规范所涉及的信号通路.
展示该平台从患者衍生的干细胞中获得hPGCLCs的实用性.

主要方法:

人类多能干细胞 (hPSCs) 在仿真植入前发育的生物工程系统中培养.
胚胎外皮样细胞 (AMLCs) 由hPSCs衍生,通过对体信号传递诱导hPGCLC的规范.
分析了包括ISL1,NODAL,WNT和BMP在内的关键信号通路的作用.

主要成果:

在生物模拟hPSC培养系统中成功诱导hPGCLCs.
来自ISL1下游的AMLCs的偏神经信号被确定为hPGCLC规范的关键驱动因素.
证实了NODAL,WNT和BMP信号在hPGCLC诱导中的功能作用.
该平台使得从八个非阻断性亚精精子症 (NOA) 患者衍生的hPSC系中获得hPGCLCs.

结论:

一个新的仿生培养系统有效地从hPSCs诱导hPGCLCs.
该系统阐明了管理hPGC规范的分子机制.
该平台在生殖医学领域的应用方面表现有前途,包括疾病建模和对不孕症原因的查.

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