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各种食脂肪酸和ETS1转录因子对SCD1表达的异位基特效应

Kinga Tibori¹, Veronika Zámbó², Gabriella Orosz¹

¹Department of Molecular Biology, Semmelweis University, 1085, Budapest, Hungary.

Scientific reports

|January 3, 2024

概括

饮食中摄入的反式脂肪酸 (TFAs),特别是埃莱达酸,会增加Stearoyl-CoA脱酶-1 (SCD1) 的表达. 一种常见的SCD1促进物多态性 (rs1054411) 也通过减少ETS1转录因子结合来提高SCD1水平.

科学领域:

分子生物学分子生物学
生物化学生化学
遗传学是一个遗传学.

背景情况:

过度营养和遗传学是代谢障碍的关键风险因素.
乙醇CoA脱酶-1 (SCD1) 调节脂肪酸合成,脂肪储存和脂毒性.
饮食中含有的反式脂肪酸 (TFAs) 和SCD1促进体多态度对SCD1表达的影响尚不清楚.

研究的目的:

为了研究酸和酸 (TFAs) 以及四种SCD1促进体多态度对SCD1表达的影响.
探索涉及转录因子 (如ETS1.1) 的监管机制.

主要方法:

使用了HEK293T和HepG2细胞培养物.
使用了Luciferase记者测定,qPCR和免疫涂抹.
对转录因子结合进行了in silico预测和in vitro验证.

主要成果:

埃莱达酸显著增加了SCD1蛋白,mRNA水平和促进剂活性,而vaccenate没有影响.
SCD1促进物多态性并没有改变基底活性,但rs1054411小等位基因增加了SCD1与各种脂肪酸的表达.
rs1054411变异减少了与SCD1促进体结合的ETS1转录因子.

结论:

埃莱达酸和rs1054411多态体是SCD1表达的新型调节剂.
通过ETS1调制对SCD1的rs1054411变体的影响需要进一步研究其在脂质代谢障碍中的作用.

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