卵泡刺激激素控制花菌细胞的谷氨酸合成,以调节排卵
Kai-Hui Zhang1,2,3, Fei-Fei Zhang1, Zhi-Ling Zhang1,2,4
1The Obstetrics & Gynecology Hospital of Fudan University, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200090, China.
Protein & cell
|January 3, 2024
概括
毛囊刺激激素 (FSH) 通过调节毛囊液 (FF) 谷氨酸水平来控制排卵. 高水平的FF谷氨酸抑制了花质细胞 (GC) 亡,这是多囊性卵巢综合征 (PCOS) 不孕症的关键因素.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 多囊卵巢综合征 (PCOS) 是由于无排卵导致的不孕症的主要原因.
- 了解驱动排卵的精确机制对于开发有效的PCOS干预措施至关重要.
研究的目的:
- 调查卵泡刺激激素 (FSH) 在控制卵泡液 (FF) 谷氨酸水平及其对排卵的影响中的作用.
- 阐明FF谷氨酸通过哪种机制影响PCOS中粒状细胞 (GC) 亡和毛囊完整性的机制.
主要方法:
- 在PCOS患者中分析FF谷氨胺水平和与排卵的相关性.
- 在小鼠中诱导PCOS特征,使用高胺的和评估GC亡.
- 研究FSH和谷氨酸对培养的小鼠毛囊破裂的影响.
- 使用甲福明或AT-101激活ASK1-JNK通路并逆转PCOS特征.
主要成果:
- 发现FSH增加了GC谷氨胺的合成,导致FF谷氨胺水平升高.
- 高FF谷氨胺通过禁用ASK1-JNK亡途径来抑制GC亡,保持毛囊壁的完整性.
- 高水平的FF谷氨酸诱导了小鼠的荷尔蒙,代谢和形态PCOS特征.
- 谷氨酸缺乏或ASK1-JNK通路激活在实验模型中逆转了PCOS特征.
结论:
- 通过FSH调节FF谷氨胺是排卵的关键决定因素.
- 谷氨胺-FSH-ASK1-JNK通路代表了管理PCOS相关不孕症的潜在治疗标.
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