在精子形成过程中,STYXL1调节了CCT复合体组合和鞭毛管的折叠
Yu Chen1,2, Mengjiao Luo1, Haixia Tu1,3
1Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, 211166, China.
Nature communications
|January 3, 2024
概括
生殖细胞特异性STYXL1对于精子运动性和男性生育能力至关重要. 它的缺失通过影响CCT复合体,损害了管素折叠和精子鞭毛发育.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
- 结构生物学 结构生物学
背景情况:
- 由管素构建的微管体构成鞭毛轴突,对精子运动和男性生育能力至关重要.
- 虽然已知轴结构,但精子鞭毛形成过程中管蛋白折叠的调节仍然不清楚.
研究的目的:
- 调查生殖细胞特异性辅助因子在调节蛋白折叠和精子鞭子发育中的作用.
- 确定参与精子生成过程中CCT复合体功能中的新型因素.
主要方法:
- 鉴定了生殖细胞特异性辅因子STYXL1,并分析了其功能.
- Styxl1淘汰赛小鼠模型是为了研究男性不孕症和鞭缺陷而生成的.
- 进行了Styxl1淘汰赛精子的蛋白质组分析.
- 研究了STYXL1和CCT复杂子单元之间的相互作用.
主要成果:
- 删除Styxl1导致男性不孕不育和精子鞭毛微管中的缺陷.
- 蛋白质组分析显示,在Styxl1淘汰精子中,鞭毛蛋白的降低调节,包括管蛋白.
- 在STYXL1的N端罗丹类域中介于与CCT复合体子单元 (CCT1,CCT6,CCT7) 的相互作用.
- 斯蒂克斯1缺乏导致CCT复合体组装受损和蛋白聚合.
结论:
- 精子细胞特异性STYXL1对于CCT促进的管氨酸折叠至关重要.
- STYXL1在精子鞭子发育和男性生育能力方面发挥着关键作用.
- 在STYXL1中存在的缺陷会破坏精子功能所必需的微管形成.
相关概念视频
01:24Microtubules in Cell Motility
3.3K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
3.3K
01:32Cytoskeletal Coordination in Cell Migration
4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.8K
01:32Microtubule Associated Motor Proteins
8.0K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
8.0K
01:02Role of Septins
1.8K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.8K
02:50Spindle Assembly
3.7K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.7K
01:32Assembly of Complex Microtubule Structures
1.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.8K