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近距离标记揭示了SQSTM1蛋白网络中的动态变化

Alejandro N Rondón Ortiz^1,2,3, Lushuang Zhang³, Peter E A Ash³

¹Department of Biology, Boston University, Boston, MA 02215, USA.

bioRxiv : the preprint server for biology

|January 3, 2024

概括

序列1 (SQSTM1) 作为自受体,形成一个动态的蛋白质网络. 这项研究揭示了聚合的蛋白如何改变SQSTM1网络,突出了参与这些相互作用的关键领域.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

序列1 (SQSTM1) 是一个关键的自受体,参与降解细胞内成分.
它的功能依赖于由特定域 (LIR,PB1,UBA,KIR) 介导的复杂蛋白相互作用网络.
了解SQSTM1网络对于理解细胞生理学和疾病发病过程至关重要.

研究的目的:

通过近距离标记来研究SQSTM1蛋白相互作用网络.
识别新型SQSTM1相互作用体,并了解聚合蛋白如何影响该网络.
阐明SQSTM1域 (PB1,UBA) 在绑定网络成员和tau.中的作用.

主要方法:

使用TurboID::SQSTM1融合蛋白进行靠近依赖生物化.
化学蛋白的细胞局部化和相互作用能力的表征.
在聚合陶的存在下分析SQSTM1互动组变化.

主要成果:

该TurboID::SQSTM1虚拟机成功地回顾了已知的SQSTM1功能,并确定了新的交互器.
聚合的蛋白显著改变了SQSTM1网络,包括与压力相关的蛋白质.
证实SQSTM1的PB1和UBA域对于绑定网络成员至关重要,包括tau的K18域.

结论:

这项研究提供了SQSTM1蛋白质网络的动态视图.
这些发现揭示了对SQSTM1互动组的影响,暗示了应激反应.
这项研究为在健康和疾病背景下研究SQSTM1提供了宝贵的资源.

关键词:

马普特 (MAPT) 是一个我们的SQSTM1是SQS.这是一个TurboIDID.自自是自的过程.在P62上,P62就是P62 蛋白质聚合蛋白质的聚合物接近性标签的标签.知道的知道的知道的

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