简单疹病毒1 (HSV-1) 使用Rab6后戈尔吉分泌途径进行病毒输出
Melissa H Bergeman1,2, Kimberly Velarde2, Honor L Glenn3
1ASU-Banner Neurodegenerative Disease Research Center, Arizona State University.
bioRxiv : the preprint server for biology
|January 3, 2024
概括
疹简单病毒1 (HSV-1) 使用宿主病毒.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 疹简单病毒1 (HSV-1) 感染了全球大多数人口,但其细胞内传输和细胞外细胞形成机制尚不清楚.
- 了解HSV-1复制的后期阶段对于开发向抗病毒疗法至关重要.
研究的目的:
- 研究涉及HSV-1细胞内贩运和外细胞形成的细胞宿主因素和途径.
- 为了描述HSV-1颗粒细胞外的确切时刻和位置.
- 为了确定HSV-1是否利用先前存在的细胞通路进行输出.
主要方法:
- 开发一种活细胞光显微镜测定方法,用于追踪HSV-1病毒贩运和外细胞形成.
- 利用pH敏感的记者来确定颗粒外细胞形成的确切地点和时间.
- 研究了HSV-1颗粒与Rab6a蛋白质的同位化和共同交易.
主要成果:
- HSV-1 颗粒利用宿主 Rab6 的高尔基后分泌途径进行输出.
- 在戈尔吉区域,HSV-1病毒与Rab6a共局,并与Rab6a囊泡向细胞外围传播.
- 通过Rab6a囊泡,HSV-1颗粒经历了外细胞分裂,这表明它依赖于先前存在的细胞通路.
结论:
- HSV-1的退出利用了宿主的先前存在的Rab6后戈尔吉分泌途径.
- 这一途径调解了HSV-1从受感染细胞中的两极分化的退出.
- HSV-1的输出不依赖于新的病毒诱导机制,而是劫持细胞机械.
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