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替代性西格玛因子RpoS通过抑制pqsABCDE操作子和激活vfr来调节Pseudomonas aeruginosa的定数感应反应

Luis Felipe Muriel-Millán¹, Luis Fernando Montelongo-Martínez¹, Abigail González-Valdez¹

¹Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, Mexico.

Molecular microbiology

|January 3, 2024

概括

伪菌中的西格玛因子RpoS通过抑制PqsE,一种稳定RhlR调节器的蛋白质,负面调节pyocyanin (PYO) 生产和生物膜形成. 这项研究澄清了RpoS.

科学领域:

微生物学微生物学
细菌病原体的产生
分子生物学分子生物学

背景情况:

伪气球菌的发病依赖于毒性因素,如皮奥 (PYO) 和生物膜形成,这些因素由定数感应 (QS) 调节.
众所周知,替代性西格玛因子RpoS会影响QS,但其在PYO生产和生物膜开发中的确切作用是复杂的,并未完全理解.
之前的研究表明,RpoS减少了lasR和rhlR转录,但它的缺失却矛盾地增强了RhlR依赖的过程.

研究的目的:

阐明RpoS在Pseudomonas aeruginosa中调节定数感应 (QS) 的机制.
调查RpoS在调节皮亚 (PYO) 生产和生物膜形成中的作用.
确定RpoS对QS监管机构的稳定性和活动的影响,特别是RhlR.

主要方法:

在不同RpoS度下分析关键QS调节者的基因转录和蛋白质水平 (RpoS,RhlR,PqsE,Vfr).
在具有不同RpoS状态的Pseudomonas aeruginosa菌株中评估pyocyanin (PYO) 生产和生物膜形成.
研究PqsE和RhlR蛋白稳定性之间的相互作用.

主要成果:

RpoS抑制了pqsE的转录,这是一种对RhlR蛋白稳定性至关重要的基因.
在没有RpoS的情况下,增加的PqsE水平稳定了RhlR蛋白,尽管rhlR转录水平降低了.
RpoS积极调节Vfr,这反过来又增强了lasR和rhlR转录,表明了双重的调节作用.

结论:

在Pseudomonas aeruginosa中,RpoS对质量检测具有负和正的监管控制.
受RpoS影响的PqsE对RhlR的稳定是控制PYO生产和生物膜形成的关键机制.
了解RpoS复杂的调节,可以了解这种机会性病原体的毒性因子控制.

关键词:

伪omonas aeruginosa 这种类型的病毒.在PqsE中使用PqsE 这是一个RhlRR.在RpoS中使用RpoS.议员数量检测是指对议员数量进行检测.

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