相关实验视频

Updated: Jul 6, 2025

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

通过纤维细胞乳酸盐,PDGF-BB加速TSCC,限制miR-26a-5p,并促进线粒

Jianguo Xu^1,2, Li Bian³, Dingyun You⁴

¹Department of Oral and Maxillofacial Surgery, Kunming Medical University School and Hospital of Stomatology, Kunming, 650106, China.

Cancer cell international

|January 3, 2024

概括

血小板衍生生长因子-BB (PDGF-BB) 驱动癌症相关纤维细胞 (CAF) 的形成,并通过改变葡萄糖代谢来促进口腔舌头状细胞癌的生长. 针对PDGF-BB和相关途径可能提供新的治疗策略.

科学领域:

在瘤学瘤学.
癌症生物学癌症生物学
分子代谢的分子代谢.

背景情况:

瘤微环境 (TME) 和与癌症相关的纤维细胞 (CAF) 在瘤进展中起着至关重要的作用.
在CAF和瘤细胞之间的代谢相互作用尚未完全理解.
口腔舌状细胞癌 (OTSCC) 是一种普遍存在的头癌.

研究的目的:

研究血小板衍生生长因子-BB (PDGF-BB) 在OTSCC中CAF形成和代谢重编程中的作用.
阐明在OTSCC TME中将PDGF-BB,CAF和糖解联系在一起的机制.
在这个途径中识别潜在的治疗点.

主要方法:

从OTSCC患者的临床数据分析.
在人口粘膜纤维细胞 (hOMFs) 的体外诱导CAF使用PDGF-BB.
评估有氧糖解,线性和自.
评估miR-26a-5p的表达及其对菌相关蛋白质的影响.
检测瘤细胞的扩散,入侵和转移.
对NF-κB信号通路激活的分析.

主要成果:

在OTSCC患者的TME中,PDGF-BB与CAF和糖解正相关.
在hOMFs中,PDGF-BB诱导了CAF表型和有氧糖解,通过线粒增强.
在CAF中观察到miR-26a-5p的下调;它的上调抑制了线粒和糖解.
PDGF-BB诱导的CAF促进了瘤细胞的增殖,入侵,转移和NF-κB的激活.
PDGF-BB与乳酸诱导的CAF形成和葡萄糖代谢重编程有关.

结论:

PDGF-BB在推动CAF形成和OTSCC中的代谢重编程方面发挥着关键作用.
线性和miR-26a-5p是PDGF-BB介导的CAF表型和甘油活性中的关键调节剂.
针对PDGF-BB/CAF轴和相关的代谢途径,为OTSCC提供了一个有前途的治疗策略.

关键词:

与癌症相关的纤维细胞.乳酸盐是一种乳酸盐.代谢重新编程的代谢.线粒细胞衰变 - - 线粒细胞衰变 (mitophagy) 是一种在PDGF-BB中.

更多相关视频

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids

Assessment of Mitochondrial Health in Cancer-Associated Fibroblasts Isolated from 3D Multicellular Lung Tumor Spheroids

Published on: October 21, 2022

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...