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Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

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基于长读数测序的细胞系中的多种HPV集成模式.

Xiaofang Cui^1,2, Yiyan Li³, Chuanpeng Zhang⁴

¹Jining Medical University, Jining, Shandong, China.

Frontiers in microbiology

|January 3, 2024

概括

人类乳头瘤病毒 (HPV) 整合到宿主基因组与宫癌有关. 长读测序揭示了多样化的HPV整合模式,证实了它在细胞系中的作用.

关键词:

在HPV的整合中,HPV的整合.在HPV16中,HPV16是最重要的.在HPV18中,HPV18是最重要的.宫癌:子宫癌是一种癌症.纳米孔测序的测序

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科学领域:

基因组学就是基因组学.
在瘤学瘤学.
病毒学病毒学

背景情况:

人类乳头瘤病毒 (HPV) 感染是导致子宫癌的主要原因.
确切的机制和HPV整合到宿主基因组的模式仍然不完全理解.
了解HPV整合对于推进宫癌诊断和治疗至关重要.

研究的目的:

研究和描述HPV整合到宿主细胞基因组的完整状态.
探索使用NANOPORE长读测序用于HPV整合分析的可行性.
在HPV阳性细胞系中识别明显的病毒整合模式.

主要方法:

在三个HPV阳性细胞系上使用NANOPORE长读测序:HeLa,SiHa和CaSki.
专门开发的软件HPV-TSD被用于分析病毒整合模式.
对不同细胞系和HPV类型 (HPV16和HPV18) 的HPV整合进行了比较分析.

主要成果:

对HPV18和HPV16观察到不同的HPV整合模式,以及HPV16整合中的变化.
CaSki细胞表现出复杂的HPV集成,而HeLa细胞显示出具有主导性的HPV18集成与不完整的病毒序列.
在HPV16和HPV18整合中发现了大量的并列重复,突出显示了复杂的整合事件.

结论:

高通量长读测序是研究HPV整合的可行方法.
该研究确定了多种HPV整合模型,证实病毒整合是HPV阳性细胞系的重要因素.
这些发现为开发HPV整合检测算法提供了基础,并为临床应用和药物开发提供了信息.