在以色列社区获得的Clostridioides difficile菌株的特征,2020-2022年
Orna Schwartz1,2,3, Hanan Rohana4, Maya Azrad4
1Azrieli Faculty of Medicine, Bar Ilan University, Safed, Israel.
Frontiers in microbiology
|January 3, 2024
概括
社区获得的Clostridioides difficile感染 (CA-CDI) 在以色列显示出高度的菌株多样性,没有单一的主导菌株. 毒素产生和生物膜形成等因素可能会影响临床结果,需要进一步调查.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 社区获得的Clostridioides difficile感染 (CA-CDI) 的流行率不断上升.
- 影响因素包括抗生素处方,高病毒性菌株和改进的诊断.
- 在以色列探索CA-CDI流行病学.
研究的目的:
- 检查以色列CA-CDI分离物的菌株多样性和毒性因子.
- 研究CA-CDI的遗传多样性和流行病学特征.
主要方法:
- 全基因组测序 (WGS) 和多位置测序类型 (MLST) 用于菌株分类和多样性分析.
- PCR和免疫测试用于检测毒素基因和蛋白质.
- 评估生物膜的产生和抗菌素敏感性.
主要成果:
- 观察到高序列类型 (ST) 的多样性,其中ST2,ST42,ST104和ST11是最常见的.
- 大多数隔离物 (92.8%) 属于Clade 1;ST与性别,地理位置或抗生素敏感性之间没有关联.
- 在ST和生物膜/毒素产生之间发现了显著的关联;观察到不同的毒素产生 (仅A,仅B,两者).
结论:
- 在C.I.C.中, 以色列的难以分离的菌株表现出高的菌株多样性,没有占主导地位的菌株.
- 毒素生产,抗生素耐药性和生物膜生产是影响临床结果的潜在因素.
- 需要进一步的研究,以了解这些因素对CA-CDI的动态和影响.
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