抗氧化剂积极调节依赖肥胖的circRNAs - 精子质量 - 功能轴
Vincenza Grazia Mele1, Teresa Chioccarelli1, Rosario Finamore1
1Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
Frontiers in endocrinology
|January 3, 2024
概括
高脂肪饮食通过氧化应激影响精子质量,损害男性生育能力. 补充生物活性分子恢复了精子circRNA配置文件和改善了运动性,这表明了与肥胖有关的不孕症的新治疗方法.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 营养科学 营养科学
背景情况:
- 肥胖引起的全身氧化应激和炎症有助于男性不孕.
- 循环RNAs (circRNAs) 是精子质量的关键调节者.
- 高脂肪饮食 (HFD) 影响精子circRNA载荷和相关网络 (ceRNETs),可能影响精子氧化应激和运动.
研究的目的:
- 研究一种生物活性分子混合物在逆转HFD诱导的男性不孕症中的有效性.
- 评估配方对HFD小鼠的circRNA生物发生和精子质量的影响.
- 阐明父亲ceRNETs在调解补充剂对精子运动的有益影响中的作用.
主要方法:
- HFD C57BL6/J雄性小鼠被口服用维生素C,B12,E,-L-甲和谷氨 (GSH) 混合物治疗了4周.
- 评估了内源性精子FUS依赖的背接机械和circRNA货物,以评估circRNA生物发生.
- 分析了精子的生命力和运动性,并通过体外检测验证了这些发现.
主要成果:
- 使用生物活性分子混合物的口服补充剂在雄性小鼠中逆转了HFD表型.
- 该配方通过影响FUS-依赖的背接来积极调节精子circRNA配置文件.
- 观察到精子活力和运动性的显著改善,与circRNA配置文件恢复相关.
结论:
- 配方中的生物活性分子有效抵消HFD引起的男性不孕症.
- 精子circRNA配置文件的恢复是补充剂对精子运动的有益影响的关键机制.
- 这些发现凸显了有针对性的营养干预措施在管理与肥胖相关的男性生殖功能障碍方面的潜力.
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