一种细菌色素提供了跨物种的保护,防止H2O2-和中性粒细胞介导的杀死
Yiwei Liu1,2, Eleanor A McQuillen3, Pranav S J B Rana1,2
1Department of Microbiology, Ohio State University, Columbus, OH 43210.
概括
Pseudomonas aeruginosa 的产物 HQNO 增加了 Staphylococcus aureus 的葡萄糖素生产. 这增强了对抗氧化应激和中性粒细胞的细菌生存,加剧了共感染.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 多微生物细菌感染,特别是 Pseudomonas aeruginosa 和 Staphylococcus aureus 的慢性共感染,带来了重大的临床挑战.
- 黄金葡萄球菌产生葡萄素 (STX),一种颜料赋予抗氧化应激和杀死中性粒细胞的耐药性.
- 了解物种间相互作用对于开发有效治疗多微生物感染至关重要.
研究的目的:
- 为了研究 Pseudomonas aeruginosa 和 Staphylococcus aureus 在多微生物感染中的相互作用.
- 确定Pseudomonas aeruginosa外产品在调节金黄色葡萄球菌毒性因子中的作用.
- 阐明Staphylococcus aureus黄金杆菌葡萄糖素在联合感染期间影响Pseudomonas aeruginosa存活的机制.
主要方法:
- 葡萄球菌黄金菌培养 (宏殖民地和浮游生物) 暴露于Pseudomonas aeruginosa外产物2-heptyl-4-hydroxyquinoline N-oxide (HQNO) 的作用.
- 在黄金葡萄球菌 (Staphylococcus aureus) 中评估葡萄球菌的生产.
- 在存在过氧化和人类中性粒细胞时评估Pseudomonas aeruginosa的存活率,有或没有金黄色葡萄球菌 (野生型和STX缺陷突变菌).
- 用野生类型和STX缺乏的金黄色葡萄球菌与Pseudomonas aeruginosa一起使用的小鼠伤口联合感染模型.
主要成果:
- Pseudomonas aeruginosa HQNO 显著诱导了 Staphylococcus aureus 中的葡萄糖素生产.
- 与野生型黄金葡萄球菌 (Staphylococcus aureus) 的共同培养中,与STX缺乏突变的单一培养或培养相比,Pseudomonas aeruginosa对过氧化和中性粒细胞的生存率得到了增强.
- 与野生型黄金葡萄球菌 (Staphylococcus aureus) 的同时感染,但不是STX缺乏突变,在小鼠伤口模型中增加了Pseudomonas aeruginosa负担和疾病严重程度.
结论:
- 伪菌HQNO通过诱导Staphylococcus aureus葡萄糖素生产来调解多微生物相互作用.
- 诱导的葡萄藻增强了黄金葡萄球菌对先天免疫因子的抵抗力,间接地促进了Pseudomonas aeruginosa的生存.
- 这项研究揭示了多微生物感染中合作毒性的机制,突出了细菌外产物在调节宿主-病原体和病原体-病原体相互作用中的作用.
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