相关实验视频

Updated: Jul 6, 2025

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Published on: April 13, 2015

作为TFEB的点,HKDC1对于维护线粒体和溶酶体平衡至关重要,防止细胞衰老

Mengying Cui¹, Koji Yamano^2,3, Kenichi Yamamoto^4,5

¹Department of Genetics, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.

Proceedings of the National Academy of Sciences of the United States of America

|January 3, 2024

概括

由转录因子EB (TFEB) 调节的含有1 (HKDC1) 的赫索金酶域,维持线粒体和溶酶体的平衡. 这种TFEB-HKDC1通路对于预防细胞衰老和器官功能障碍至关重要.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

线粒体和溶酶体功能障碍与细胞衰老,衰老和疾病有关.
控制线粒体和溶酶体平衡的精确调节机制仍然不完全理解.
转录因子EB (TFEB) 是 lysosomal 功能的关键调节者,在线粒体调节中扮演着新兴的角色.

研究的目的:

阐明TFEB维持线粒体和溶酶体平衡的机制.
为了确定参与器官维护的新型TFEB目标.
研究HKDC1在细胞应激反应和衰老中的作用.

主要方法:

综合转录组分析以确定TFEB目标.
染色体免疫沉-qPCR用于验证TFEB-HKDC1相互作用.
功能性试验评估HKDC1在线粒细胞衰变,溶酶体修复和衰老中的作用.

主要成果:

确定HKDC1是直接TFEB目标,在线粒体和溶酶体应激下以TFEB依赖的方式进行上调.
TFEB-HKDC1轴对PINK1/帕金因依赖的线粒和溶酶体修复至关重要.
HKDC1保持了线粒体-溶解体接触,并防止DNA损伤诱导的细胞衰老.

结论:

在TFEB的下游,HKDC1的作用是维持线粒体和溶酶体的平衡.
TFEB-HKDC1通路在预防细胞衰老方面发挥着至关重要的作用.
在器官维护中的HKDC1的功能是独立于其在糖解中的作用.

关键词:

一个HKDC1是HKDC1.美国联邦税务法 (TFEB)细胞衰老细胞衰老线粒体溶酶体接触接触线粒细胞衰变 (mitophagy) 是一种神经衰变的过程.

更多相关视频

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Published on: March 28, 2025

相关概念视频

Inhibition of Cdk Activity

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...