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超高通量,实时流动细胞计,用于从全血中定量罕见细胞

Mahmut Kamil Aslan¹, Yingchao Meng¹, Yanan Zhang^2,3

¹Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Vladimir-Prelog-Weg 1, Zürich 8093, Switzerland.

|January 3, 2024

概括

这项研究引入了一种新的超高通量光细胞计,用于实时细胞分析. 该系统能够快速检测罕见事件,例如循环瘤细胞 (CTC),具有高灵敏度.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
微流体学微流体学

背景情况:

传统的流细胞计在吞吐量和罕见事件检测方面面临局限性.
精确量化循环瘤细胞 (CTC) 对癌症诊断和监测至关重要.

研究的目的:

开发一个超高通量,实时光细胞计.
为了证明其对敏感的罕见事件检测能力,特别是外周血液中的CTC.

主要方法:

粘弹性微流体系统与CMOS线性图像传感器的集成.
开发一个定制的C++控制程序和GUI用于实时数据处理.
在周围血液中应用患者衍生的CTC量化系统.

主要成果:

实现了每秒超过40万种的分析吞吐量.
在罕见事件检测方面表现出高灵敏度 (每百万血细胞中6个CTC).
在CTC分析中实现了超过3毫升/分钟的体积吞吐量.

结论:

开发的光细胞计为细胞分析提供了前所未有的吞吐量和灵敏度.
这个平台显示出作为早期癌症检测和患者监测的临床工具的巨大潜力.
该系统可以实时量化罕见细胞,提高诊断能力.

关键词:

循环中的瘤细胞.流动细胞计量是流动细胞计量的方法.光是一种光.高通量的高通量微流体学在微流体学方面实时实时的时间.

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