LncRNA STAT3-AS通过STAT3信号通路调节子宫内膜受体
Yanni Jia1, Wei Wang2, Jiaqi Jiang1
1College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, China.
Theriogenology
|January 3, 2024
概括
羊的子宫内膜受容性对于怀孕至关重要. 发现一个长非编码RNA,STAT3-AS,通过调节STAT3通路并促进胚胎附着,对这一过程至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 分子内分泌学分子内分泌学
背景情况:
- 在反动物中,子宫内膜受容性对于成功怀孕至关重要.
- 干扰素 (IFNT) 激活JAK/STAT通路,诱导干扰素刺激基因 (ISG) 进行胚胎附着.
- 一种新的长非编码RNA,STAT3-AS,在由IFNT.调节的山羊子宫内膜上皮细胞 (gEECs) 中被确定.
研究的目的:
- 研究STAT3-AS在山羊中建立子宫内膜受体性的作用和调节机制.
- 为了阐明STAT3-AS在IFNT介导的信号通路中的参与.
主要方法:
- 在各种荷尔蒙条件下和怀孕期间,对gEECs中STAT3-AS表达的定量分析.
- 短干扰RNA (siRNA) 在gEECs中介于STAT3-AS的淘汰.
- 评估ISG,与子宫内膜受体性相关的基因和gEEC增殖.
- 使用特定激活剂 (RO8191) 调查STAT3通路的激活.
主要成果:
- 在怀孕期间以及对IFNT,孕激素和雌激素的反应中,STAT3-AS表达在子宫上调.
- STAT3-AS knockdown显著抑制了关键ISG和子宫内膜受体性标记物的表达 (HOXA11,ITGB3,VEGF).
- STAT3-AS缺陷抑制了gEEC增殖和STAT3通路激活,可以通过STAT3激活来挽救.
结论:
- 在山羊中,STAT3-AS是子宫内膜受容性的关键调节剂.
- STAT3-AS通过STAT3信号通路调解其功能,影响ISG表达和细胞增殖.
- 向STAT3-AS可能为提高反动物的生殖效率提供了一种新的策略.
相关概念视频
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Hormonal Regulation of the Menstrual Cycle
396
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
396
Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
Regulation of Expression at Multiple Steps
914
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
914
MicroRNAs
21.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.3K


