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相关概念视频

CRISPR01:59

CRISPR

51.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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相关实验视频

Updated: Jul 6, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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辛卡斯:高效的CRISPR/Cas9基因编辑在难以转化的关节动物中.

Sander De Rouck1, Antonio Mocchetti1, Wannes Dermauw1

  • 1Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium.

Insect biochemistry and molecular biology
|January 3, 2024
PubMed
概括

研究人员优化了CRISPR/Cas9传递用于节肢动物基因组编辑,使用一种名为SYNCAS的新配方. 这种方法显著提高了基因淘汰效率,例如双斑蜘蛛和西部花.

关键词:
在ABC-运输商.这就是BAPC.在Cas9的交付中,Cas9的交付.基合成酶是一种基合成酶.内体逃生试剂的内体逃生试剂假基因是一种假基因.

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科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 昆虫学 昆虫学是一门学科.

背景情况:

  • CRISPR/Cas9是一种强大的基因组编辑工具,在关节动物研究中具有广泛的应用.
  • 传统的微注射方法对许多节肢动物物种来说是低效和具有挑战性的,包括双斑蜘蛛 (Tetranychus urticae).

研究的目的:

  • 优化CRISPR/Cas9的输送,以便在T. urticae.中有效地注入母体.
  • 开发一种新的CRISPR/Cas9配方,以提高难以转化关节动物的基因组编辑效率.

主要方法:

  • 探索各种CRISPR/Cas9配方用于T. urticae的孕产妇注射.
  • 开发和测试一个协同配方,结合分支的两原性囊和素 (SYNCAS).
  • 应用SYNCAS用于基因淘汰,共同CRISPR策略和T. urticae中的淘汰突变以及Frankliniella occidentalis中的基因淘汰.

主要成果:

  • 一种新的CRISPR/Cas9配方SYNCAS证明了协同效应,显著提高了T. urticae的淘汰效率,达到20%以上.
  • 在T. urticae中,SYNCAS启用了向基因淘汰 (植物脱酶,CYP384A1,Antennapedia),共同CRISPR和淘汰突变基因生成.
  • 在西方花 (Frankliniella occidentalis) 中成功应用SYNCAS对基因淘汰.

结论:

  • 这种SYNCAS配方代表了CRISPR/Cas9在关节动物中的突破,克服了以前的局限性.
  • 这种方法在以前具有挑战性的物种中促进了常规基因组编辑,并有可能在关节动物遗传研究中得到更广泛的应用.