表皮层-半机体过渡标记在阿托皮性皮肤炎的表皮层表达
Kazuyuki Kitazawa1, Kazunori Tanaka1, Yoshiki Kubota1
1Pharmaceutical Products Research and Development Section, NANOEGG® Research Laboratories, Inc.
Biological & pharmaceutical bulletin
|January 3, 2024
概括
皮肤上皮质-介质细胞过渡 (EMT) 标志物在亚托皮炎 (AD) 皮肤中升高,表明在这种炎症性皮肤疾病中可能发挥作用. 这些变化甚至发生在非损伤性皮肤中,这表明在AD病变发生过程中存在类似EMT的过程.
科学领域:
- 皮肤病学 皮肤病学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 表皮-介质细胞过渡 (EMT) 对于伤口愈合至关重要,但过度的EMT会导致组织功能障碍.
- 介质素 (IL) - 4和IL-13,在亚托皮性皮肤炎 (AD) 中涉及的细胞因子,可以诱导EMT.
- 亚托皮炎的特点是炎症,和湿疹.
研究的目的:
- 为了调查在皮肤上的EMT的存在和意义的阿托皮性皮肤炎模型和患者.
- 探索EMT与AD病变发生之间的潜在联系.
主要方法:
- 在AD小鼠模型和人类AD皮肤样本中分析EMT标志物表达 (Snai1,Twist,Vimentin).
- 对上皮细胞标记物 (克拉5) 和混合上皮细胞-介质细胞 (E/M) 细胞标记物 (ΔNp63a) 的评估.
- 对阿兹海默症患者的非损伤性皮肤中的EMT标记表达的检查.
主要成果:
- 在AD小鼠模型和患者中观察到EMT标记Snai1和Twist的表达增加.
- 在AD皮肤表皮中,表皮标记蛋白5和介质标记蛋白Vimentin的同时表达表明混合E/M细胞.
- 在AD小鼠皮肤中发现了混合E/M细胞稳定剂ΔNp63a的升调.
- 即使在非损伤性AD皮肤中,也检测到升高的EMT标志物,独立于炎症或痕.
结论:
- 类似于EMT的现象存在于亚托皮炎患者和模型的皮肤中.
- 杂交的上皮细胞-介质细胞可能在AD的发病过程中发挥作用.
- 在阿尔茨海默病的皮肤中,类似于EMT的变化可能独立于伤口愈合过程而发生.
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