卵泡刺激激素触发了猪细胞中线粒体结构和功能的快速变化
Amel Lounas1, Yann Breton2, Ariane Lebrun1
1Centre de recherche en reproduction, développement et santé intergénérationnelle (CRDSI), Département des sciences animales, Faculté des Sciences de l'agriculture et de l'alimentation, Université Laval, Québec, QC, G1V 0A6, Canada.
Scientific reports
|January 3, 2024
概括
毛囊刺激激素 (FSH) 改变了猪卵细胞周围细胞中的线粒体,影响了能量使用. 增加的循环腺单酸盐 (cAMP) 防止在成熟过程中有害的线粒体碎片化.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 线粒体动力学的动力学
背景情况:
- 卵细胞成熟对女性生育能力和胚胎发育至关重要.
- 累积细胞通过转移由FSH和cAMP调节的代谢物来支持卵细胞.
- 线粒体在卵细胞成熟中的作用尚未完全理解.
研究的目的:
- 研究FSH和升高的cAMP对猪细胞中的线粒体动力学和功能的影响.
- 了解线粒体对卵细胞成熟过程的贡献.
主要方法:
- 来自猪的积卵细胞复合体 (COC) 的体外成熟 (IVM).
- 观测线粒体结构 (延长/碎片化) 和活动.
- 在IVM培养前对细胞内cAMP水平的操纵.
主要成果:
- 在累积细胞中,FSH诱导了线粒体的延长,随后在24小时后发生碎片化.
- 线粒体延长与线粒体活动减少和糖分分解增加相关.
- 在IVM培养前细胞内cAMP升高可以防止线粒体碎片化.
结论:
- 在COC中,FSH迅速改变线粒体结构和功能.
- 这些变化是由cAMP依赖的途径介导的.
- 线粒体动力学对于成功的卵细胞成熟至关重要.
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