Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Next-generation Sequencing03:00

Next-generation Sequencing

88.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A comparative analysis of promoter-proximal pausing reveals kinetic and distributional dimensions of variation.

bioRxiv : the preprint server for biology·2026
Same author

Benchmarking large language models for cell-free RNA diagnostic biomarker discovery.

Nature communications·2026
Same author

Functional and computational interrogation of juvenile idiopathic arthritis risk loci in CD4+ T cells.

HGG advances·2026
Same author

Meiotic prophase I disruption as a strategy for nonhormonal male contraception using small-molecule inhibitor JQ1.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

An end-to-end generalizable deep learning framework to comprehensively analyze transcriptional regulation.

Nature communications·2026
Same author

Smoothie: efficient inference and integration of spatial co-expression networks from denoised spatial transcriptomics data.

Communications biology·2026
Same journal

The penicillin-binding protein PBP1b fortifies the Escherichia coli division site against osmotic rupture.

Nature microbiology·2026
Same journal

Identification of chemical features for improved outer membrane permeation in mycobacteria using machine learning.

Nature microbiology·2026
Same journal

Author Correction: Gut commensal Christensenella minuta modulates host metabolism via acylated secondary bile acids.

Nature microbiology·2026
Same journal

Mobile genetic elements shape microbial diversity and functions in thawing permafrost soils.

Nature microbiology·2026
Same journal

Epistatic interactions inform rational design of synthetic microbial communities for bioremediation.

Nature microbiology·2026
Same journal

END nucleases are antiphage defence systems targeting multiple phages with modified genomes.

Nature microbiology·2026
查看所有相关文章

相关实验视频

Updated: Jul 6, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.5K

微生物组转录组学的精确运行测序 (PRO-seq).

Albert C Vill1, Edward J Rice2, Iwijn De Vlaminck3

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.

Nature microbiology
|January 3, 2024
PubMed
概括
此摘要是机器生成的。

精确运行测序 (PRO-seq) 描述了各种细菌的新兴RNA转录,包括未培养的微生物. 这种方法揭示了RNA聚合酶 (RNAP) 暂停动机和人类微生物群中RNAs的并发转录分裂.

更多相关视频

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

19.7K
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

38.7K

相关实验视频

Last Updated: Jul 6, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.5K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

19.7K
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
12:37

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

38.7K

科学领域:

  • 微生物基因组学和转录基因组学
  • 细菌的基因调节 细菌的基因调节
  • 大基因组学和微生物组分析.

背景情况:

  • 细菌通过转录启动和延长来调节基因表达.
  • 大量RNA测序捕获成熟的转录,缺少活跃转录的区域.
  • 新生的RNA捕获方法仅限于可培养的细菌.

研究的目的:

  • 应用精确运行测序 (PRO-seq) 来对包括未培养物种在内的多种细菌的新生转录进行分析.
  • 描述小,结构化或改性RNA的转录,这些RNA被大量RNA测序遗漏.
  • 研究人类微生物组中的转录动态和RNA聚合酶 (RNAP) 行为.

主要方法:

  • 使用精密运行测序 (PRO-seq) 来捕获与新生RNA复杂的RNA聚合酶 (RNAP).
  • 该方法应用于培养的大肠杆菌和各种未培养的细菌.
  • 来自人类微生物组的PRO-seq数据被分析为RNAP暂停动机和RNA处理.

主要成果:

  • 在培养和非培养细菌中,PRO-seq成功地对新生转录进行了分析.
  • 该技术确定了小型,结构化和修改RNA的转录,这些RNA通常不在散装RNA-seq.中.
  • 对人类微生物组的分析揭示了特定类型的RNAP暂停动机和CRISPR指导RNA和tRNA的并发转录分裂.

结论:

  • PRO-seq是一个多功能工具,用于研究跨多种细菌群体的新生转录.
  • 该方法提供了对小RNA和修饰RNA的转录的洞察.
  • PRO-seq阐明了转录动态,包括RNAP暂停和RNA处理,在复杂的微生物群落,如人类微生物群.