相关实验视频

Updated: Jul 6, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

对乳腺癌的Notch转录密码签名的识别

Eike-Benjamin Braune¹, Felix Geist², Xiaojia Tang³

¹Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

Breast cancer research : BCR

|January 3, 2024

概括

研究人员开发了一种20个基因的签名来测量乳腺癌中的Notch信号. 这种工具可以监测Notch-targeting疗法并评估当前治疗的效果,帮助乳腺癌治疗的患者分层.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

失调的Notch信号与乳腺癌的进展有关.
缺乏有效的工具来量化乳腺癌亚型和治疗期间的Notch信号水平.
为了监测Notch-targeting疗法并调查当前治疗的非目标效应,需要一个转录组签名.

研究的目的:

为了建立一个转录学签名来测量乳腺癌中的Notch信号.
为了能够监测Notch信号的响应系统治疗.
为了确定乳腺癌治疗对Notch信号的潜在非目标效应.

主要方法:

在基底类乳腺癌细胞系中鉴定了Notch调控基因,这些细胞系具有改变的Notch信号.
开发了使用Notch调节基因的候选转录基因签名.
在乳腺癌患者和细胞系数据集 (TCGA-BRCA,METABRIC,Oslo2) 上训练并验证的签名.

主要成果:

选择了一种最佳的20基因转录组签名,具有更好的连贯性和瘤特异性.
在基底类乳腺癌中,签名得分最高,这表明Notch信号的提升.
签名得分在新辅助治疗后增加,较低的得分与更好的临床结果相关.

结论:

这20个基因的签名作为一个有价值的工具,用于评估对未来的Notch-targeting疗法的反应.
它可以帮助理解传统乳腺癌治疗对Notch信号的影响.
签名有助于更好的患者分层治疗考虑.

关键词:

基底类类似的基底类.乳腺癌是什么? 乳腺癌是什么乳腺癌的干细胞是什么?诊断诊断诊断诊断没有口的信号.治疗疗法治疗疗法治疗耐药性治疗耐药性转录的签名转录的签名三重负的三重负.

更多相关视频

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

The Nucleolus

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...