相关实验视频

Updated: Jul 6, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

一种新的现场处理技术可以构建整个圆形cpDNA库

Qiang Zhou¹, Xianlong Ding¹, Hongjie Wang¹

¹Key Laboratory of Biology and Genetics Improvement of Soybean, Ministry of Agriculture of the People's Republic of China, Zhongshan Biological Breeding Laboratory (ZSBBL), National Innovation Platform for Soybean Breeding and Industry-Education Integration, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, National Center for Soybean Improvement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Soybean Research Institute, College of Agriculture, Nanjing Agricultural University, Nanjing, China.

|January 3, 2024

概括

一种新的in-situ技术有效地提取圆形叶绿体DNA (cpDNA),并创建有序的库. 这种方法有助于植物遗传学和叶绿体基因组研究.

科学领域:

植物基因组学植物基因组学
分子进化的分子进化.
人类基因组学是什么?

背景情况:

叶绿体基因组 (cp基因组) 对于植物分子基因组和进化研究至关重要.
cp基因组具有高度可塑性,存在各种大小和形状.
丰富循环叶绿体DNA (cpDNA) 简化了cp基因组研究,有序的DNA库比无序的更实用.

研究的目的:

开发一种使用目标插入cpDNA片段构建有序BAC库的技术.
提高基因组学研究的cpDNA库建设的效率和质量.

主要方法:

一种新的 in-situ 工艺技术,结合了 in-situ 叶绿体溶解和 MCE 膜上的 in-situ 替代物/结合.
优化了质细胞溶解的缓冲器和程序,与细菌溶解不同.
构建目标插入,有序的cpDNA库,防止大插入片段断裂.

主要成果:

从作物中有效提取循环cpDNA,并建立高质量的cpDNA库.
成功创建了目标插入,有序的cpDNA库,这些库排列在微型板上.
图书馆包含明确的cpDNA片段,与整个圆形cpDNA相同.

结论:

这种新的in-situ技术显著推进了全基因组功能分析和质细胞特征的研究.
促进叶绿体基因组测序,生物信息学分析,克隆,物理映射,以及分子类遗传学和进化研究.

关键词:

在BAC图书馆的图书馆里.塑是一种塑.一个圆形cpDNA.在现场溶解.在现场的替代品/结合.在现场处理过程中.

更多相关视频

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

相关概念视频

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

PCR

PCR