相关实验视频

Updated: Jul 6, 2025

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

通过TET2介导的ECM1低甲基化促进了活性增殖性糖尿病视网膜病变的新血管化

Chunyang Cai^1,2, Chufeng Gu^1,2, Shuai He^1,2

¹Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Hongkou District, Shanghai, 200080, People's Republic of China.

Clinical epigenetics

|January 3, 2024

概括

在糖尿病视网膜病变 (DR) 中失调的TET2通过减少像ECM1.1这样的基因的DNA甲基化来促进新血管化. 向TET2为增殖性糖尿病视网膜病变 (PDR) 提供了一种新的治疗方法.

科学领域:

分子生物学分子生物学
眼科医生眼科眼科
遗传学遗传学是一个

背景情况:

甲基酸二氧化酶2 (TET2) 在糖尿病视网膜病变 (DR) 中高度表达.
高TET2水平降低了DNA甲基化,激活了与视网膜毛细血管泄漏和高葡萄糖环境中的新血管化相关的基因转录.
了解TET2的下游基因对于调查多发性糖尿病视网膜病变 (PDR) 至关重要.

研究的目的:

为了在PDR中识别TET2调节的下游基因.
调查TET2和PDR中的新血管化之间的关联.
探索针对TET2.2的治疗潜力.

主要方法:

对公共数据集 (GSE60436,GSE57362,GSE158333) 的分析,以确定 PDR 中的 TET2 相关基因.
在高葡萄糖处理的人类视网膜微血管内皮细胞 (HRMECs) 中验证基因表达和促进物甲基化.
TET2敲击实验评估其对HRMEC功能和下游基因表达的影响.

主要成果:

确定了PARVB,PTPRE和ECM1作为关键的TET2相关基因.
高葡萄糖增加了TET2和ECM1表达,同时降低了HRMECs中的ECM1促进物甲基化.
通过TET2倒置减少HRMEC迁移和管形成,伴随着减少PARVB,PTPRE,ECM1表达和增加ECM1促进物甲基化.

结论:

失调的TET2通过改变下游基因甲基化和转录,特别是ECM1.1,促进PDR新血管化.
基因促进体的TET2诱导的低甲基化是PDR的潜在治疗标.
这项研究为PDR中新血管化的机制提供了新的见解.

关键词:

通过DNA甲基化.在ECM1中使用ECM1.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.新血管化的新血管化.扩散性糖尿病视网膜病变 (PDR)在TET2的基础上.

更多相关视频

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Published on: October 7, 2018

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Assessment of Vascular Regeneration in the CNS Using the Mouse Retina

Published on: June 23, 2014

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...