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来自Trichinella spiralis的盖莱克丁通过调节肠道微生物群来缓解小鼠的DSS诱导的大肠炎
Jianqing Li1,2,3, Xiangjiang Wang2, Qiuhui Wang1,2,3
1School of Pharmacy, Fujian Medical University, Fuzhou, 350004, Fujian, China.
Veterinary research
|January 4, 2024
概括
再组合的Trichinella spiralis galectin (rTs-gal) 通过抑制炎症通路和调节肠道微生物群来改善小鼠的实验性结肠炎,为炎症性肠病 (IBD) 提供潜在的治疗益处.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
- 寄生虫学的寄生虫学
背景情况:
- 三螺旋菌 (T. spiralis) 抗原调节宿主免疫反应以维持恒温.
- T. spiralis galectin是一种具有潜在免疫调节活性的蛋白质.
- 重组T. spiralis galectin (rTs-gal) 对硫酸 (DSS) 诱导的大肠炎的治疗作用尚不清楚.
研究的目的:
- 调查rTs-gal改善小鼠实验性结肠炎的能力.
- 探索rTs-gal在炎症性肠病 (IBD) 中治疗作用的潜在机制.
主要方法:
- 在小鼠中使用DSS诱导大肠炎.
- 用rTs-gal来评估治疗功效.
- 使用疾病活动指数 (DAI),结肠缩短和组织学损伤评估临床症状.
- 通过西式涂抹和ELISA分析炎症标志物 (TLR4,MyD88,NF-κB).
- 评估肠道微生物群组成和脂多糖 (LPS) 生产.
主要成果:
- rTs-gal的使用显著改善了DSS诱导的大肠炎的临床症状.
- rTs-gal抑制了结肠中TLR4,MyD88和NF-κB的诱导.
- rTs-gal调节肠道微生物群,减少产生LPS的细菌,如Helicobacter和Bacteroides.
- rTs-gal抑制了涉及LPS,TLR4和NF-κB的信号通路.
结论:
- 再组合的T. spiralis气质素 (rTs-gal) 显示出对DSS诱导的大肠炎的治疗潜力.
- rTs-gal通过抑制炎症信号通路和调节肠道微生物群来起作用.
- 这些发现凸显了虫衍生蛋白质作为IBD新型治疗方法的潜力.
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