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Updated: Jul 6, 2025
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A Macrophage-Tumor Spheroid Co-Invasion Assay
Published on: January 24, 2025
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综合蛋白质学分析揭示了瘤相关巨细胞及其前体细胞在瘤进展中的动态表型
Ying Liu1, Di Liu1, Yuchen Liu1
1State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China.
Journal of proteome research
|January 4, 2024
概括
这项研究揭示了瘤相关巨细胞 (TAM) 在癌症进展过程中如何发生变化. 单细胞 (Mons) 在进入瘤微环境 (TME) 之前失去炎症潜力,影响TAM发育.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 蛋白质组学是指蛋白质组学.
背景情况:
- 与瘤相关的巨细胞 (TAMs) 在癌症进展中至关重要.
- 骨髓衍生单细胞 (Mons) 作为TAM前体的确切作用及其TME调节的表型尚未完全理解.
研究的目的:
- 研究TAM及其单细胞前体的动态表型.
- 描述不同黑色素瘤进展阶段的TAMs和Mons的蛋白质表达特征.
主要方法:
- 开发了一种针对细胞数量较少的髓状细胞的优化微蛋白学工作流.
- 从小鼠黑色素瘤模型中分类的TAM和Mons (1 × 10^5细胞/样本).
- 使用质谱学分析了蛋白质表达.
主要成果:
- 在瘤进展过程中确定了一种TAM表型,具有上调的胆固醇代谢和下调的免疫反应.
- 在晚期TAM和Mons.中观察到STAT5和PYCARD的下调.
- 在晚期的Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.Mons.
结论:
- 单细胞前体在进入瘤微环境之前就会失去炎症潜力.
- 提供了关于TAM和前体细胞在瘤进展过程中的转变的见解.
- 突出了瘤和骨髓之间的跨器官通信.
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