一个eudicot MIXTA家族的祖先很可能在形细胞和三体细胞中发挥作用
Simra Zahid1, Anjelique F Schulfer1, Verónica S Di Stilio1
1Department of Biology, University of Washington, Seattle, WA, United States.
Frontiers in plant science
|January 4, 2024
概括
在Thalictrum中,古MIXTA (PMX) 基因家族在开发植物表皮特征,如叶子三角体和花细胞中发挥着关键作用. 这项研究揭示了PMX.
科学领域:
- 植物发育生物学植物发育生物学
- 进化遗传学的进化遗传学
- 分子植物科学 分子植物科学
背景情况:
- MYB转录因子的MIXTA家族调节了各种植物表皮发育.
- 像Thalictrum这样的早期分离的尤迪科特提供了对祖先基因功能的洞察.
- R2R3 SBG9-A MYB转录因子,称为"古MIXTA" (PMX),代表了核心前尤迪科特的重复.
研究的目的:
- 调查Thalictrum中MIXTA基因家族的进化史和功能作用.
- 描述Thalictrum paleoMIXTA (ThPMX) 在表皮发育中的功能.
- 为了重建古米克斯塔血统在尤迪科特的祖先功能.
主要方法:
- 在23个Thalictrum物种中对ThPMX正统的克隆和遗传学分析.
- 定量PCR (qPCR) 用于不同植物组织的表达分析.
- 病毒诱导的基因沉默 (VIGS) 和烟草中的异质过度表达用于功能特征.
- RNA-Seq转录组分析以确定下游的调节途径.
主要成果:
- 确定了ThPMX基因的全系复制,与全基因组复制事件相吻合.
- 在 carpels 中观察到最高的 ThPMX 表达,在叶子中也发现了显著的表达.
- 功能性表征表明,ThPMX (TthPMX) 调节叶子三胞体的发育和形态发生,可能是通过与微管相关的机制.
- 有证据表明,paleoMIXTA的正确基因在叶子三体和花圆状乳头细胞中起着双重作用.
结论:
- 古老MIXTA基因家族在Thalictrum属中经历了重复和多样化.
- thalictrum paleoMIXTA的 ортолог参与了叶子三合体和花表皮细胞的发展.
- 这一发现支持了paleoMIXTA基因在调节表皮细胞命运 (包括三细胞发育) 方面保留的祖先功能.
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