Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Uni-portal thoracoscopic lymph node dissection under the left prominence: pause ventilation technique.

Journal of cardiothoracic surgery·2026
Same author

Clinical study on the therapeutic effect of large-area cupping therapy for lumbar disc herniation of cold-damp type (a Traditional Chinese Medicine syndrome).

Frontiers in medicine·2026
Same author

Bakuchiol, a potentially toxic component in Fructus psoraleae, causes liver damage in vitro by affecting the homeostasis of the liver GABA signalling system.

Journal of ethnopharmacology·2026
Same author

Rosmarinic acid-derived micelles potentiated dextran hydrogel for concurrent mechanical reinforcement and inflammatory microenvironment remodeling in chronic diabetic wound healing.

Carbohydrate polymers·2026
Same author

Isolation and characterization of a highly pathogenic Orthoflavivirus associated with ascites syndrome in Chinese soft-shelled turtles (Pelodiscus sinensis).

Archives of virology·2026
Same author

A Personalized FSH Dosing Strategy for Women with Polycystic Ovary Syndrome Undergoing GnRH Antagonist Protocols.

Biomedicines·2026

相关实验视频

Updated: Jul 6, 2025

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

15.7K

基于卷积神经网络的快速数字重定焦里埃图形显微镜方法.

Mingdi Liu, Ruofei Wu, Zicong Luo

    Optics express
    |January 4, 2024
    PubMed
    概括

    这项研究引入了一种新的卷积神经网络,通过准确预测失焦距离来提高福里埃图形显微镜 (FPM) 图像质量. 这种方法提高了成像深度,并在各种生物样本中保持高精度.

    科学领域:

    • 显微镜的使用方法
    • 计算成像技术的成像
    • 生物医学光学 生物医学光学

    背景情况:

    • 里埃图形显微镜 (FPM) 提供高分辨率和大视野成像.
    • 传统的FPM重建与焦点外的文物作斗争,降低了图像质量.
    • 准确的失焦估计对于强大的FPM图像重建至关重要.

    研究的目的:

    • 开发一种用于FPM中准确估计失焦距离的新方法.
    • 为了提高图像质量,并扩展FPM系统的成像深度.
    • 为了解决传统的FPM重建在缺焦的情况下的局限性.

    主要方法:

    • 设计了一个使用卷积神经网络 (CNN) 的失焦距离回归网络.
    • 该网络使用实验FPM数据进行了训练和验证.
    • 定量评估涉及计算预测和真实的失焦距离之间的根平均平方误差 (RMSE).

    主要成果:

    • 提出的基于CNN的方法实现了约6.2μm的低RMSE,用于失焦距离预测.
    • 该方法在不同的生物样本中展示了强大的概括能力.
    • FPM系统的有效成像深度被扩展了超过3倍的因素.

    更多相关视频

    Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
    11:12

    Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

    Published on: December 1, 2021

    2.0K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    17.7K

    相关实验视频

    Last Updated: Jul 6, 2025

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
    11:34

    High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

    Published on: December 3, 2013

    15.7K
    Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
    11:12

    Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

    Published on: December 1, 2021

    2.0K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    17.7K

    结论:

    • 开发的失焦距离回归网络显著提高了FPM图像重建质量.
    • 这种方法提供了一个强大的解决方案,用于处理FPM中的失焦问题.
    • 该方法为生物样本分析提供了更好的成像性能和更深的场域.