快速检测耐药性Mycobacterium结核病通过修改的MODS测定适合资源贫困的设置
Bashir Ahamd Fomda1, Gulnaz Bashir1, Sehrish Baqal1
1Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, India.
PLoS neglected tropical diseases
|January 4, 2024
概括
一种修改的显微观测药物敏感性 (MODS) 试验可以减少结核病检测的污染和成本. 这种用户友好的方法可以在低收入环境中早期诊断结核病和耐药性.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 诊断检测试验 诊断检测试验
背景情况:
- 微观观察药物敏感性 (MODS) 测定带来了交叉污染和生物安全问题的风险.
- 为了应对这些挑战,对MODS技术进行了修改.
研究的目的:
- 评估一种改进的MODS技术,以改善结核病检测和药物敏感性测试.
- 评估修改后的MODS试验的生物安全性,成本效益和周转时间 (TAT).
主要方法:
- 在组织培养瓶中对275个样本进行了修改的MODS.
- 药物敏感性测试使用间接阿加比例方法和基因型MTBDRplus测试.
- 与LJ媒体和传统方法进行了比较.
主要成果:
- 修改后的MODS显示出对结核病检测的高灵敏度 (97.44%) 和特异性 (80.00%).
- 观察到异化和利芬素敏感性测试的良好一致性 (kappa = 0.923和1分别).
- 与固体介质相比,修改后的MODS显示污染率降低,成本降低,TAT更快.
结论:
- 修改后的MODS试验具有成本效益,易于使用,并最大限度地降低了处理人员的风险.
- 在修改后的MODS技术中没有观察到交叉污染.
- 这种修改后的测定方法适用于在资源有限的环境中早期检测结核病和耐药性.
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