相关实验视频

Updated: Jul 6, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

通过ADAR介导的RNA编辑调节了结直肠癌中的PVR免疫检查点

Cheng-Jia Qian¹, Yu-Shan He², Tao Guo²

¹Department of General Surgery, Affiliated Hospital of Jiangnan University, Wuxi, China; Laboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, 1800 Lihu Avenue, Wuxi, China.

Biochemical and biophysical research communications

|January 4, 2024

概括

在结直肠癌 (CRC) 中,ADAR介导的RNA编辑可调节脊髓灰质炎受体 (PVR) 表达的表达. 这一发现为CRC提供了潜在的新诊断生物标志物,改善了对免疫疗法耐药性的理解.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

瘤免疫疗法耐药性与ADAR介导的RNA编辑有关.
这种RNA编辑在癌症中的具体目标在很大程度上仍然未知.

研究的目的:

在结直肠癌 (CRC) 中确定ADAR介导的RNA编辑的目标.
研究脊髓灰质炎病毒受体 (PVR) 在CRC进展中的作用及其通过RNA编辑的调节.
评估PVRRNA编辑作为CRC的诊断生物标志物.

主要方法:

在两个中国CRC队列中进行了转录组序列分析.
实验验证包括基因表达分析和基于细胞的测试 (过度表达,淘汰).
露西法酶记者和阿克丁诺米辛D测定以评估RNA稳定性和表达调节.

主要成果:

在CRC瘤中,PVR和ADAR表达显著增加.
在CRC瘤中观察到升级的PVRRNA编辑,与PVR和ADAR表达呈正相关.
在CRC细胞中,ADAR操纵改变了PVR表达和RNA编辑.
在PVR 3'-UTR中编辑RNA可以增强PVRRNA的表达,很可能是通过增加RNA的稳定性.
组合PVRRNA编辑和表达的签名在CRC中显示出有前途的诊断性能.

结论:

通过ADAR介导的RNA编辑在CRC中对PVR进行上调方面发挥着至关重要的作用.
编辑PVRRNA代表了CRC的一个潜在的新型诊断生物标志物.
这些发现为RNA编辑对CRC瘤和免疫功能的贡献提供了新的见解.

关键词:

结肠直肠癌是一种癌症.诊断诊断诊断诊断诊断免疫检查点连接体.脊髓灰质炎病毒受体受体编辑RNA的RNA编辑

更多相关视频

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...