相关实验视频

Updated: Jul 6, 2025

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

黄胺4,4'-dimethoxychalcone通过激活ferritinophagy选择性地消除衰老细胞

Tianxiang Wang¹, Changmei Yang¹, Zhiqiang Li¹

¹MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, PR China.

|January 4, 2024

概括

4,4'-dimethoxychalcone (DMC) 通过诱导铁亡,可以选择性地消除衰老细胞. 这种黄类化合物显示出作为抗衰老剂对抗与年龄有关的疾病的前景.

科学领域:

生物化学生物化学
老年学是指老年学的学科.
细胞生物学细胞生物学

背景情况:

黄酸是具有健康益处的天然化合物.
4,4'-dimethoxychalcone (DMC) 是一种黄类化合物,之前已经证明它通过抑制铁甲基酶 (FECH) 来诱导铁.
在此之前,DMC对细胞衰老的影响是未知的.

研究的目的:

为了研究DMC对细胞衰老的影响.
阐明DMC影响衰老细胞的机制.
评估DMC作为老化剂的潜力.

主要方法:

用DMC,奎尔塞丁和达沙替尼治疗衰老细胞.
在衰老细胞与非衰老细胞中识别FECH表达.
涉及抑制FECH,ferritinophagy和不稳定铁池的机制研究.
在老老鼠体内研究,以评估DMC对与年龄相关的表型的影响.

主要成果:

DMC选择性地消除了老化的细胞.
在衰老的细胞中,FECH的表达很高.
DMC抑制FECH,诱导ferritinophagy,增加可变铁,并触发老化的细胞中的ferroptosis.
在老小鼠中,DMC治疗可以预防脱发,改善运动协调,并减少与衰老相关的分泌表型因子.
与DMC和奎尔丁或达沙替尼布的联合治疗在衰老细胞清除方面表现出高效率.

结论:

DMC是一种强大的老化剂,可以通过铁死选择性地消除老化的细胞.
DMC的机制涉及FECH的抑制,导致费里丁和铁的积累.
DMC在预防与年龄相关的病理和改善健康方面显示出治疗潜力.

关键词:

4,4′-二次氧化基子这里是 FECH FECH 的位置.费里丁诺巴基 (Ferritinophagy) 是一种对铁素的消化.铁化是铁化的一种.衰老是一种衰老.年老的药物.

更多相关视频

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

Published on: February 23, 2024

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Published on: September 13, 2022

相关概念视频

Electron Transport Chain: Complex I and II

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...

Phagocytosis of Apoptotic Cells

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...