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Updated: Jul 6, 2025

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在细菌细胞表面进行分裂-GFP补充,用于无抗体标记和异质蛋白显示量的量化

David Gercke1, Florian Lenz1, Joachim Jose1

  • 1Universität Münster, Institut für Pharmazeutische und Medizinische Chemie, PharmaCampus, Corrensstrasse 48, 48149 Münster, Germany.

Enzyme and microbial technology
|January 4, 2024
PubMed
概括

研究人员开发了一种新方法,使用分裂绿色光蛋白 (GFP) 技术在细菌上光标记表面蛋白质. 这种技术可以精确量化和表征全细胞生物催化剂,而不会影响其活性.

关键词:
自动显示器自动显示.细胞表面 细胞表面流动细胞计量流动细胞计量蛋白质标签的使用方法蛋白质的量化量化方法分拆-GFP的时间.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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