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系统评估标准储存条件对废水样本中等离子体结合行为的影响

Yuxi Yan¹, Xiang Li², Kaiqiang Yu²

¹School of Environment, Harbin Institute of Technology, Harbin 150001, China; School of Environmental Science and Engineering, College of Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Environmental pollution (Barking, Essex : 1987)

|January 4, 2024

概括

样品储存对等离子体结合和抗生素耐药性基因转移产生重大影响. 最佳条件对于准确的环境抗性研究至关重要,特别是对于有影响力的样本.

科学领域:

环境微生物学环境微生物学
等离子体生物学
抗生素耐药性抗生素耐药性

背景情况:

过器配对实验对于研究环境样本中的等离子体结合和抗生素耐药性至关重要.
样品储存条件 (温度和持续时间) 对这些实验的影响尚不清楚.
标准储存温度 (4°C,-20°C,-80°C) 对它们对等离子体结合的影响进行了评估.

研究的目的:

系统地研究不同储存温度和持续时间如何影响等离子体结合效率.
评估储存对环境样本 (影响和活性污泥) 中抗体组成的影响.
为准确的抗体研究提供最佳样本处理指南.

主要方法:

过器配对实验是在污水处理厂 (STP) 的流入物 (Inf) 和活性污泥 (AS) 样本上进行的.
样品在标准条件下存储:4°C,-20°C和-80°C,存储时间不同.
在不同的存储场景下,分析了等离子体结合效率和抗体基因型.

主要成果:

在所有测试的储存条件下,结合效率显著下降,除了在4°C储存1周外.
与-20°C相比,在-80°C的储存在活性污泥中更好地保持结合,但对流入样本的有效性较低.
影响性样本显示IncH型等离子体的损失和抗性基因型的下降趋势,存储时间在4°C和-20°C增加.

结论:

样品储存条件极大地影响了等离子体结合行为和抵抗体组成.
活性污泥样本在储存条件下显示出比影响样本更统一的抗体样本.
仔细考虑储存温度和持续时间对于可靠的环境耐药性研究至关重要.

关键词:

在 ARG 中,有 ARG 的存在.微生物社区的微生物社区.等离子体的结合.等离子体不兼容性等离子体不兼容性储存条件储存条件储存条件

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