人类IgG与Streptococcus pyogenes中M相关蛋白之间的相互作用的分子表征
Emma-Jayne Proctor1, Hannah R Frost2, Sandeep Satapathy3
1Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, Australia.
The Journal of biological chemistry
|January 4, 2024
概括
尽管序列多样化,A组链球菌M相关蛋白 (Mrps) 结合了人类IgG亚类,具有纳米分子亲和力. 所有的Mrps对IgG3的结合都较弱,影响了宿主-病原体相互作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 结构生物学 结构生物学
背景情况:
- 甲组链球菌M相关蛋白 (Mrps) 是对细菌毒性至关重要的表面蛋白.
- Mrps通过其A重复区域结合人体免疫球蛋白G (IgG),有助于细菌的生存.
- 在Mrps中,序列的多样性引发了关于它们保存的IgG结合功能的疑问.
研究的目的:
- 调查Mrps的序列多样性是否影响它们与人类IgG子类的相互作用.
- 为了描述Mrp-IgG相互作用的结合亲和力和固基度.
主要方法:
- 使用表面等离子体共振 (SPR) 来量化Mrp-IgG亚类的结合亲和力.
- 血拉下测试,其次是西式涂抹测试,评估了复杂血清中的IgG结合.
- 确定了Mrp-IgG相互作用的静脉测量.
主要成果:
- 不同的Mrps对人类IgG亚类 (3.711.1nM对混合IgG) 具有纳米分子亲和力.
- 与其他IgG子类相比,所有Mrps对IgG3的结合明显较弱.
- Mrps以1:1的胆固醇测量结合IgG,并在其他血清蛋白质存在时保持结合.
结论:
- 尽管有序列的变化,但Mrps保持了对人类IgG子类的高亲和度结合.
- 对IgG3的优先结合和较弱的亲和力表明它在宿主免疫逃避中发挥了特定的作用.
- 1:1体积测量可以更清楚地了解这种关键的宿主-病原体相互作用.
关键词:
一组A型链球菌M 蛋白质家族的蛋白质家族.与M相关的蛋白质是M相关的蛋白质.链球菌 pyogenes 的情况.结合性亲和力是一种结合性亲和力.具有约束力的静电测量.免疫球蛋白G 免疫球蛋白G 是一种病变的发生和发病.分类偏好子类的偏好.毒性 毒性是一种毒性.更多相关视频
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