通过控制BNIP3的营业额,ER膜蛋白复合体限制了 mitofgy
Jose M Delgado1, Logan Wallace Shepard1, Sarah W Lamson1
1Department of Biochemistry and Cell Biology, Geisel School of Medicine, Dartmouth College, Hanover, NH, USA.
The EMBO journal
|January 4, 2024
概括
线受体BNIP3和BNIP3L/NIX绕过自,使用另一种途径进行 lysosomal 输送. 这种内体体贩运调节了线粒和尾部固的蛋白质定位.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 自学研究 自学研究
背景情况:
- 选择性自,包括线性自,通常通过自受体的溶酶体降解来评估.
- 像BNIP3和BNIP3L/NIX这样的线粒体受体对向线粒体进行降解至关重要.
- 了解调节这些受体的精确机制是理解选择性自的关键.
研究的目的:
- 为了研究线粒体受体BNIP3和BNIP3L/NIX的溶酶体降解途径.
- 确定涉及到BNIP3走私和退化中的新因素.
- 阐明不同细胞降解系统在调节BNIP3稳态中的相互作用.
主要方法:
- 全基因组的CRISPR屏幕用于识别影响BNIP3流动的因素.
- 对自独立的溶酶体输送通路的分析.
- 在BNIP3调控中研究内酶体和无素-蛋白酶体系统的作用.
主要成果:
- BNIP3和BNIP3L/NIX是独立于自的组成性地传递给 lysosomes.
- 包括ER膜蛋白复合体 (EMC) 在内的内溶性体组件对于BNIP3溶性体贩运至关重要.
- 内分泌体和无素-蛋白酶体系统独立调节BNIP3,而扰乱其中任何一个会影响线粒和细胞生理学.
结论:
- BNIP3的溶解体降解通过一种自独立的内分体途径发生.
- 内体体贩运和 lysosomal 降解是尾部定蛋白质局部化的关键调节者.
- 这些发现扩大了对尾部定蛋白质质量控制和选择性自机制的理解.
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