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Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
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基于LC-MS的方法的比较,用于在动物基质中确定碳素酸

Heidi E Schwartz-Zimmermann1,2, Manuel Hündler3,4,5, Nicole Reiterer3,4,6

  • 1Department of Agrobiotechnology, IFA-Tulln, Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences, Vienna (BOKU), Tulln, Austria. heidi.schwartz@boku.ac.at.

Analytical and bioanalytical chemistry
|January 4, 2024
PubMed
概括

建议使用3-Nitrophenylhydrazine (3-NPH) 衍生来量化动物样本中的碳酸 (CA),使用逆相液态染色学-并联质谱法 (RP-LC-MS/MS). 氨酸衍生显示出较低的回收率和不准确的量化,使其不适合这些分析.

关键词:
阳离子交换色谱学 阳离子交换色谱学衍生化 衍生化 衍生化质谱测量质量谱测量逆相高性能液态染色学 逆相高性能液态染色学短链脂肪酸 短链脂肪酸

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In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
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