在口腔状细胞癌中,TIMELESS促进了葡萄糖代谢的重编程
Yafan Chen1, Zhengyang Han2, Le Zhang3
1Department of Nuclear Medicine, Tangdu Hospital, Air Force Medical University, 569 Xinsi Road, Xi'an, 710038, Shaanxi, China.
Journal of translational medicine
|January 4, 2024
概括
昼夜基因TIMELESS通过增强糖解和减少氧化酸化,主要通过SIRT1通路,促进口腔状细胞癌 (OSCC) 的生长. 准无时间性为OSCC提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 循环节生物学 循环节生物学
背景情况:
- 口腔状细胞癌 (OSCC) 的发病率高,存活率低.
- 循环节律的破坏与癌症的发展有关.
- 昼夜基因TIMELESS在OSCC中的作用还没有得到充分研究.
研究的目的:
- 研究TIMELESS对OSCC细胞生长和代谢变化的影响.
- 探索OSCC中TIMELESS,SIRT1和代谢途径之间的关系.
主要方法:
- 分析了OSCC组织和细胞系中的TIMELESS表达,使用西部斑块,IHC,qRT-PCR,TCGA和CCLE数据.
- 评估了TIMELESS在OSCC扩散,细胞周期和瘤生长中的作用.
- 评估了TIMELESS对细胞代谢的影响,包括葡萄糖摄取,乳酸生产,氧气消耗和关键代谢蛋白 (SIRT1,HK2,PKM2,LDHA,GLUT1).
主要成果:
- 时间表达在OSCC中升高,与患者存活时间减少相关.
- 无时无刻的过度表达增强了OSCC细胞的增殖和糖解,同时抑制了氧化酸化.
- 无时间敲击扭转了这些效应,无时间表达与SIRT1,HK2,PKM2,LDHA和GLUT1积极相关.
结论:
- 无时无刻地加剧OSCC的进展,通过促进糖解和减少由SIRT1通路介导的氧化酸化.
- 无时间性代表了OSCC的潜在治疗目标.
相关概念视频
02:49Cancer Therapies
7.7K
Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
7.7K
02:53Adaptive Mechanisms in Cancer Cells
5.8K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.8K
01:29Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
01:22PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
01:26Oral Hypoglycemic Agents: Biguanides and Glitazones
206
Biguanides, particularly metformin (Glucophage), are insulin sensitizers that enhance glucose uptake, thereby reducing insulin resistance. Unlike sulfonylureas, metformin doesn't prompt insulin secretion, which helps to curb hypoglycemia risk. Metformin is beneficial in treating conditions like polycystic ovary syndrome due to its insulin-resistance reduction capability. The drug's primary action involves curtailing hepatic gluconeogenesis, a significant contributor to high blood...
206
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
7.4K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.4K