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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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多路径工件使多式内镜光学连贯断层扫描中的角度对比成为可能.

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    此摘要是机器生成的。

    光学连贯断层扫描 (OCT) 中的多路径器件可以重新使用. 这些文物揭示了高角度反射的特征,使得新的多路径对比成像能够从现有的OCT数据中获得.

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    科学领域:

    • 生物医学光学 生物医学光学
    • 在光学成像系统中,光学成像
    • 光纤光学是指光纤的使用.

    背景情况:

    • 多路径器件在双层光纤连贯性断层扫描 (OCT) 中很常见.
    • 这些来自更高阶模式的工件通常会降低内镜多式联接OCT系统中的图像质量.
    • 以前被认为是一种限制,它们的潜在效用尚未被探索.

    研究的目的:

    • 研究光学连贯断层扫描 (OCT) 中多路径器件的潜力.
    • 为了证明这些文物可以用来生成高质量的面对面图像,编码高角度背向散射.
    • 引入多路径对比作为一种新的成像模式.

    主要方法:

    • 以实验和模拟为基础,将散射器合成基本和工件模式的表征.
    • 使用内镜多模式OCT系统对微球进行成像.
    • 开发多路径对比作为差异合的比度测量.

    主要成果:

    • 多路径文物可以投射到高质量的二维面部图像中.
    • 这些图像显示了Mie范围散射器的高角度反射特征.
    • 多路径对比提供了关于样本角度多样性的信息,并且可以从现有的OCT数据中生成.

    结论:

    • 多路径工件在OCT不仅仅是有害的,可以用于成像.
    • 多路径对比为分析样本角度多样性提供了一种新方法.
    • 这种技术允许对大量的海洋和海上国家的临床数据进行回顾性分析.