在进化过程中保存的Wnt/Sp5信号对海胚胎前后轴模式至关重要
Sujan Gautam1, Jennifer L Fenner1, Boyuan Wang1
1Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
iScience
|January 5, 2024
概括
Wnt/Sp5信号录音带对于在海胚胎中建立初级生殖层至关重要. 这种古老的信号通路在后部内皮和前部神经外皮的模式中起着关键作用.
科学领域:
- 发展生物学 发展生物学
- 进化发育生物学 进化发育生物学
- 分子生物学分子生物学
背景情况:
- Wnt信号通路是元动物发育的基础.
- 转录因子Sp5经常被Wnt信号激活,形成一个保存的信号磁带.
- 在早期生殖层形成和轴模式中Wnt/Sp5信号传递的作用在所有deuterostomes中并未完全理解.
研究的目的:
- 为了研究Wnt/Sp5信号在初级生殖层规范和前后轴模式在海胚胎中的功能.
- 阐明Sp5在正规 (Wnt/β-catenin) 和非正规 (Wnt/JNK) Wnt通路下游的特定作用.
- 为了比较 Wnt/Sp5 信号传递在海中的功能,与其它双胞胎体的功能.
主要方法:
- 在海胚胎中使用基因操纵的功能分析.
- 表达模式分析.表达模式分析.
- 对 Wnt/Sp5 信号跨越双胞胎体物种进行比较分析.
主要成果:
- 在Wnt/β-catenin信号传导的下游,sp5对于后部海胚胎细胞的内皮特异性至关重要.
- Sp5对于前部神经外皮的模式至关重要,是非正规Wnt/JNK信号的下游.
- 功能和表达数据揭示了Wnt/Sp5信号在deuterostomes的后部和前部模式中的保留角色.
结论:
- Wnt/Sp5信号录音带在子胚组胚胎的生殖层规范和前后轴图案中发挥着至关重要的,保存的作用.
- 这些发现支持这样一个假设:Wnt-Sp5信号传递是常见deuterostome祖先中生殖层建立的组成部分.
- 这项研究强调了Wnt/Sp5信号在动物早期发育中的进化意义.
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