相关实验视频

Updated: Jul 6, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

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针对Streptococcus pyogenes A类酶A的P1'含有Leu的标序列的独特结合模式导致了替代裂解

Brandon A Vogel1, Jadon M Blount1, Hanna M Kodama1

  • 1Department of Chemistry, Western Washington University, 516 High St - MS9150 Bellingham WA 98225 USA antosj@wwu.edu amachej@wwu.edu +1-360-650-2826 +1-360-650-2271 +1-360-650-4397.

RSC chemical biology
|January 5, 2024
PubMed
概括

格拉姆阳性细菌中的Sortase A (SrtA) 酶可以在标准LPXTG动机之外进行分裂. 这项研究揭示了Streptococcus pyogenes SrtA中的另一种结合方式,使新的蛋白质结合应用成为可能.

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