针对性下一代测序用于肺部感染诊断在不适合支气管支气管洗的患者
Zhenfeng Deng1, Chunhong Li1, Yingjin Wang2
1Clinical Genome Center, Guangxi KingMed Diagnostics, Nanning, China.
Frontiers in medicine
|January 5, 2024
概括
与传统方法相比,目标下一代测序 (tNGS) 为肺部感染提供了优越的病原体检测. 这种快速诊断技术有助于指导抗生素治疗,并揭示特定年龄的病原体差异.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 微生物学 微生物学
- 传染性疾病 传染性疾病
背景情况:
- 肺部感染带来了重大的诊断挑战.
- 传统的微生物学测试 (CMT) 在识别广泛的病原体方面存在局限性.
- 目标下一代测序 (tNGS) 是一种新兴的传染病快速诊断技术.
研究的目的:
- 用唾液样本评估tNGS在诊断肺部感染方面的临床疗效.
- 为了比较tNGS与CMT的诊断产量.
- 评估tNGS对临床决策和抗生素治疗的影响.
主要方法:
- 从209名确诊肺部感染的儿童和成人患者中采集了唾液样本.
- 在唾液样本上进行了tNGS分析.
- 临床数据被追溯分析,tNGS结果与CMT进行了比较.
主要成果:
- tNGS检测到的病原体 (45) 比CMT (23) 多得多,总检测率为96.7%与36.8% (p < 0.001).
- tNGS的结果指导了21名患有罕见或高度致病性微生物的患者的诊断和治疗.
- 根据tNGS发现,对38.8%的患者进行了治疗调整,这也揭示了年龄特定的病原体分布.
结论:
- 唾液样本的tNGS为潜在的病原体提供了有价值的见解,增强了肺部感染的诊断,特别是在无法进行支气管支气管洗的患者中.
- tNGS提供了更高的检测率,并且比CMT更有效地影响临床管理.
- 该研究强调了tNGS在识别各种病原体和告知向治疗方面的有用性.
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