通过探索来自希腊的葡萄酵母微生物群来开发酒精发酵启动剂的新选择方法
Aikaterini P Tzamourani1, Vasileios Taliadouros2, Ioannis Paraskevopoulos1
1Department of Wine, Vine and Beverage Sciences, School of Food Science, University of West Attica, Athens, Greece.
Frontiers in microbiology
|January 5, 2024
概括
这项研究使用分子和表型方法确定了土著希腊葡萄酒酵母,主要是Saccharomyces cerevisiae. 它提出了一种新的生物统计方法,用于有效选酒潜力,帮助葡萄酒的质量.
科学领域:
- 葡萄酒学和葡萄种植
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 本地酵母对于成功的酒精发酵和葡萄酒质量至关重要.
- 了解本土酵母的多样性和酒潜力对于酒至关重要.
- 主导葡萄酒酵母物种的地理分布和特征需要进一步调查.
研究的目的:
- 根据酒潜力创建和分类一系列新的酵母分离物.
- 为了探索Saccharomyces cerevisiae在希腊的地理分布.
- 开发一种快速选土著葡萄酒酵母的方法.
主要方法:
- 分子鉴定 (RAPD,MALDI-TOF MS) 和表型特征 (杀伤性毒素,代谢物产生,SO2耐药性).
- 使用delta-PCR进行Saccharomyces cerevisiae的菌株类型化.
- 实验室规模的发酵和葡萄酒的感官评估.
- 生物统计工具,包括等级集群分析.
主要成果:
- 收集了190个酵母分离物,其中Saccharomyces cerevisiae是最占主导地位的物种.
- 非Saccharomyces的物种,如Trigonopsis californica和Brettanomyces bruxellensis在较低的丰度中被确定.
- 鉴定了20种Saccharomyces cerevisiae菌株,其中65%显示出发酵能力,导致干葡萄酒.
- 现型分析显示,大多数隔离品中都存在中性杀毒毒素敏感性和低酸产量.
结论:
- 一种新的生物统计方法使得本土葡萄酒酵母的有效选和分类成为可能.
- 该研究证实了基于表型指纹和感官评估的酵母分类.
- 这种方法有助于更有效地预选出具有酒学前景的酵母分离物.
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