在从HBV感染到肝细胞癌的进展过程中,m7G相关的LncRNAs的转录组范围动态
Min Shi1, Shunshun Zhu1, Linying Sun2
1Department of Pathophysiology, School of Basic Medical Sciences, Weifang Medical University, 261053 Weifang, Shandong, China.
Frontiers in bioscience (Landmark edition)
|January 5, 2024
概括
N7-甲基瓜诺辛 (m7G) 修改影响B型肝炎病毒 (HBV) 介导的肝癌 (HCC) 中的长非编码RNA (lncRNAs). TEKT4P2和DNM1P41 lncRNAs显示m7G依赖的表达,在HCC发展中提供潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 在瘤学瘤学.
背景情况:
- 在RNA功能中N7-甲基瓜诺辛 (m7G) 修饰的作用正在出现.
- 在乙型肝炎病毒 (HBV) 相关的肝细胞癌 (HCC) 发展期间对长非编码RNA (lncRNAs) 的影响基本上是未知的.
研究的目的:
- 研究 lncRNA m7G 修饰在 HBV 介导的 HCC 中的调控作用.
- 在肝癌发生过程中识别具有动态m7G变化的特定lncRNA.
主要方法:
- MeRIP-seq被用来比较来自HCC组织的lncRNAs中的m7G修饰水平,邻近的准癌组织和正常的肝脏组织.
- 在正交分析中,发现了具有一致的m7G变异的lncRNAs.
- 在体外实验验证实了这些发现.
主要成果:
- 在比较准癌和正常组织时,856个lncRNA显示了改变的m7G修饰.
- 1775个lncRNA在HCC和准癌组织之间发生了差异性修饰.
- 六个lncRNAs表现出一致的超-m7G修饰,其中TEKT4P2和DNM1P41表现出m7G依赖的表达.
结论:
- 这项研究全面分析了HBV-HCC. lncRNA m7G修饰的HBV-HCC.
- 在HCC进展过程中,多个lncRNA经历m7G变化.
- TEKT4P2和DNM1P41被确定为HBV介导的HCC的潜在分子标.
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