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OMIP-100:一个流细胞测量面板来研究人类中性粒细胞子集

Craig J Schofield¹, Rabindra Tirouvanziam^2,3, Luke W Garratt^1,4

¹Wal-Yan Respiratory Research Centre, Telethon Kids Institute, University of Western Australia, Nedlands, Western Australia, Australia.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

|January 5, 2024

概括

这项研究引入了一种多功能多色免疫光面板,用于深度中性粒细胞子集分析. 这种工具有助于了解不同的人类样本中中性粒细胞在健康和疾病中的可塑性.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

中性粒细胞是具有多种功能和可塑性的关键免疫细胞.
了解中性粒细胞子集对于诊断和治疗各种疾病至关重要.

研究的目的:

开发和验证一个14色,13个抗体优化的多色免疫光面板 (OMIP).
为了使各种人类样本类型中中性粒细胞子集的深度分析.
为了促进中性粒细胞可塑性在健康和病态的背景.

主要方法:

面板设计的重点是细胞内和细胞表面标记物,用于中性粒细胞的发育,迁移,细胞化,颗粒释放和免疫调节.
光素分配优先考虑常见的可用选择 (FITC/AF488,PE,APC) 对于关键标记物,如Olfactomedin 4,CD10和CD177.
在全血和纯化的中性粒细胞上进行了优化,在临床样本和体外转移试验中证明了应用.

主要成果:

OMIP允许对中性粒细胞子集进行详细的表征.
该面板是可适应的,关键标记可以替换,用于探索新的抗原或功能.
从全血,唾液和体外刺激样本分析中性粒细胞的证明有用性.

结论:

开发的OMIP为分析中性粒细胞可塑性提供了一个统一的平台.
这种工具有望促进对中性粒细胞子集在健康和疾病中的理解.
促进对中性粒细胞异质性和不同生物环境中的功能进行研究.

关键词:

颗粒释放的颗粒可以释放.颗粒细胞是颗粒细胞.免疫调节的免疫调节移民移民迁移迁移作为一个中性恋者.人类的本体生长过程 (ontogeny)发酵细胞的形成发酵细胞的形成这是一个子集子集.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...