相关实验视频

Updated: Jul 6, 2025

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

一个简单的和通用的对DNA和RNA的Tandem Oligonucleotide合成方法

Jagandeep S Saraya¹, Derek K O'Flaherty¹

¹Department of Chemistry, University of Guelph, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada).

Chembiochem : a European journal of chemical biology

|January 5, 2024

概括

使用新型链接器的双重寡核酸合成 (TOS) 提高了DNA/RNA合成效率. 这种方法可以创建复杂的核酸结构,包括siRNA,具有修改链接的潜力.

科学领域:

生物化学生物化学
有机化学有机化学
分子生物学分子生物学

背景情况:

自动化寡核酸合成对于分子生物学和治疗学至关重要.
当前的方法面临复杂结构的效率和可扩展性的局限性.
串联寡核酸合成 (TOS) 提供了一种有前途的方法来克服这些挑战.

研究的目的:

推出一种新的,商业上可用的链接器,用于高效的Tandem Oligonucleotide Synthesis (TOS).
为了证明这种方法对于DNA和RNA合成的可通用性.
展示TOS在组装复杂的核酸结构和结合修改的链接中的应用.

主要方法:

使用商业上可用的2,2'-硫乙烯连接剂,通常用于酸化.
将链接器纳入TOS的寡核酸序列.
执行了标准的脱保护步骤,以触发链接器的自焚.
组装了一个模型小干扰RNA (siRNA) 结构.
证明了模板导向的结合,用于结合胺酸和酸盐结合.

主要成果:

在标准的DNA和RNA脱保护过程中,2,2'-硫乙烯连接器有效地自焚.
这种方法被证明是核酸TOS的通用化方法.
通过使用TOS策略成功组装了一个模型siRNA构造.
展示了一个模板导向的结合路径,用于将非自然链接 (胺酸,酸盐) 纳入DNA寡合体.

结论:

2,2'-硫乙烯连接器为Tandem Oligonucleotide合成提供了一种高效和可通用的方法.
这种TOS策略增强了像siRNA这样复杂的核酸结构的组装.
该方法促进了修改链接的结合,扩大了核酸化学的工具包.

关键词:

化学化试剂化学化试剂在DNA/RNA合成过程中.N3′→P5′光胺酸DNA合成酸链接的DNA与酸相关.双重组的寡核酸合成

更多相关视频

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

相关概念视频

Lagging Strand Synthesis

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...

Complementary DNA

Complementary DNA

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

DNA Replication

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...