温度影响鼻上上皮细胞共同培养模型中的共生病原体动态
Joshua T Huffines1, RaNashia L Boone1, Megan R Kiedrowski1
1Division of Pulmonary, Allergy, and Critical Care, Department of Medicine, Heersink School of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, USA.
mSphere
|January 5, 2024
概括
温度在上呼吸道 (URT) 中显著影响金黄色葡萄球菌和Corynebacterium物种之间的相互作用. 较低,健康的URT温度会抑制S. aureus的生长并减少上皮损伤,而较高,疾病的温度会促进失生症.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 病变的发生和发病.
背景情况:
- 慢性鼻炎 (CRS) 涉及鼻的炎症性疾病,由微生物失调驱动.
- 黄金葡萄球菌 (Staphylococcus aureus) 是上呼吸道 (URT) 中常见的病原体,经常在CRS患者中发现.
- 菌种,包括C.propinquum和C.pseudodiphtheriticum,在健康的URT中是丰富的.
研究的目的:
- 研究温度在URT中开始性,致病性细菌和上皮细胞动态中的作用.
- 了解温度如何影响Corynebacterium物种和S. aureus在健康与病态环境中的相互作用.
主要方法:
- 利用空气-液体接口细胞培养模型来模拟鼻上皮质环境.
- 在不同的温度下检查了细菌殖民,持久性,聚合物形成和上皮细胞毒性,代表健康和患病的URT条件.
- 分析了Corynebacterium物种和S. aureus之间的单种和双种相互作用.
主要成果:
- 模仿健康URT的温度促进了共生Corynebacterium的持久性和聚合,同时降低了S. aureus的生长和上皮细胞毒性.
- 较高的温度,类似于患有CRS鼻的温度,加剧了黄金色细菌的生长和细胞毒性.
- 在双物种殖民期间,在Corynebacterium物种和S. aureus之间观察到温度依赖的,特定物种的相互作用.
结论:
- 上呼吸道粘膜温度是调节多微生物和宿主细菌相互作用的关键因素.
- 温度变化可能会在慢性上呼吸道疾病 (如CRS) 中显著导致微生物失调.
- 了解温度的作用对于开发针对CRS的向治疗策略至关重要.
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