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来自Bacteroidota的多糖体利用位点编码CE15酶,它们可能在裂开pectin-lignin键中发挥作用

Andrea Seveso¹, Scott Mazurkewich¹, Sanchari Banerjee²

¹Wallenberg Wood Science Center, Division of Industrial Biotechnology, Department of Life Sciences, Chalmers University of Technology, Gothenburg, Sweden.

Applied and environmental microbiology

|January 5, 2024

概括

碳水化合物雌激酶家族15 (CE15) 酶,在多糖化物利用位点 (PUL) 中发现,可以在pectins中切割尿酸,而不仅仅是素-xylan键. 这项研究研究了植物细胞壁解构中的新型CE15功能.

科学领域:

生物化学生物化学
植物细胞壁结构植物细胞壁结构
酶学是一种酶学.

背景情况:

由于氨酸-多糖化合物的共价链接,氨酸的回火阻碍了酶性水解.
建议使用葡萄糖烯酸乙酶 (CE15) 通过裂解氨酸-葡萄糖烯酸乙键来减少反性.
一些CE15酶在多糖利用位点 (PUL) 中被发现,除了葡萄糖基之外的目标不明.

研究的目的:

调查5名CE15成员的生物作用,这些成员来自非向葡萄糖氧的PUL.
描述这些CE15酶在模型基质和新型pectin-lignin模仿物的活性.
确定一个代表性的CE15酶中结合联体的结构基础.

主要方法:

生物信息分析以确定特定PUL中的CE15成员.
使用葡萄糖乙酶模型基质和合成pectin-lignin模仿剂的酶分析.
用X射线晶体学来确定具有和没有连接体的CE15酶的结构.
对富含点氨酸的生物质进行酶活性测定.

主要成果:

从PUL中特征了五种CE15酶.
对*Phocaeicola vulgatus*CE15酶的结构分析显示,与葡萄糖酸盐相比,银河酸氨酸具有明显的结合.
对于研究的CE15酶,没有观察到pectin甲基化酶的活性.
有证据表明CE15酶可能会在rhamnogalacturonans中切割尿酸.

结论:

针对pectin的PUL中的CE15酶可以将尿酸分成复杂的pectin,如rhamnogalacturonan II.
这项研究为CE15酶的新功能提供了洞察力,超越了氨酸-烯酸键裂变.
这项研究有助于了解植物细胞壁的复杂化学成分和潜在的生物质解构策略.

关键词:

碳水化合物雌激酶的使用碳水化合物雌激酶家族15的15个葡萄糖烯酸乙酶是什么纤维素酸盐是什么纤维素酸盐是什么质素 (pectin) 是一种质素.蛋白质结构蛋白质结构

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